UASt regulatory sequences drive expression of an inverted repeat.
Adult flies expressing ManfGD4793 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4Hml.Δ show more extensive eye degeneration after exposure of one eye to UV irradiation during pupal stage compared to wild-type.
Expression of ManfGD4793 under the control of Scer\GAL4repo, in combination with a Dicer-2 transgene to enhance RNAi efficiency, causes lethality at third instar larval stage; these third instar larvae also have a locomotion phenotype with reduced peristaltic contraction frequency, circular path trajectories and a lack of climbing compared to control larvae. Expression of ManfGD4793 under the control of Scer\GAL4repo, in the absence of Dicer-2, does not cause larval lethality and does not affect the number of dopaminergic neurons in any studied cluster (PAL, PPL1, PPL2ab, PMM1/2 or PPM3) of the adult brain. Expression of ManfGD4793 under the control of Scer\GAL4elav.PLu or Scer\GAL4ple.PF does not cause significant differences in the number of dopaminergic neurons within any studied dopamine neuron cluster, but sporadic absence of dopamine neuron clusters can occur.
Adults expressing ManfGD4793 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Expression under the control of Scer\GAL4pnr-MD237 results in lethality before the pupal stage or semi-lethality at the pupal stage, depending on the insertion line used.