FlyBase Allele Report: Dmel\Arl2[GD12240]

General Information

Symbol

Dmel\Arl2^GD12240

Species

D. melanogaster

Name

FlyBase ID

FBal0198753

Feature type

allele

Associated gene

Dmel\Arl2

Associated Insertion(s)

Carried in Construct

P{GD12240}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD12240}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD12240

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | third instar larval stage, with Scer\GAL4^insc.PU

(Chen et al., 2016)

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^insc.PU

(Chen et al., 2016)

increased cell number | somatic clone | third instar larval stage, with Scer\GAL4^ase.neuro

(Chen et al., 2016)

increased cell number | somatic clone | third instar larval stage, with Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

(Chen et al., 2016)

increased cell number | third instar larval stage, with Scer\GAL4^insc.PU

(Chen et al., 2016)

lethal, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

partially lethal - majority die, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

larval neuroblast | increased number | third instar larval stage, with Scer\GAL4^insc.PU

(Chen et al., 2016)

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

larval neuroblast | somatic clone | increased number | third instar larval stage, with Scer\GAL4^ase.neuro

(Chen et al., 2016)

larval neuroblast | somatic clone | increased number | third instar larval stage, with Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

(Chen et al., 2016)

spindle | third instar larval stage, with Scer\GAL4^insc.PU

(Chen et al., 2016)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

type II neuroblast | somatic clone | third instar larval stage, with Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

(Chen et al., 2016)

type I neuroblast | somatic clone | third instar larval stage, with Scer\GAL4^ase.neuro

(Chen et al., 2016)

Detailed Description

Statement

Reference

Expression of Arl2^GD12240 driven by Scer\GAL4^insc.PU results in supernumerary neuroblasts in the third instar larval central brain and .

Arl2^GD12240 clones have extra neuroblasts in both type I (Scer\GAL4^ase.neuro) and II (Scer\GAL4^wor.PA and Scer\GAL80^ase.PN) neuroblast lineages, compared to a single neuroblast in control clones (third instar larval central brain).

(Chen et al., 2016)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in loss of neuroblasts in the larval brain associated with decreased size of the remaining neuroblasts.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in a reduction in the average size of neuroblasts together with shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in a decrease in the average size of ganglion mother cells in the larval brain compared to controls.

(Neumüller et al., 2011)

Adults expressing Arl2^GD12240 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in viable flies or late pupal lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0%, 80-90% or 100% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development