UASt regulatory sequences drive expression of an inverted repeat.
Adults expressing tankGD892 under the control of Scer\GAL4tub.PU (expression restricted to the adult stage using the temperature sensitive Scer\GAL80ts.αTub84B allele) show increased resistance to ethanol sedation compared to controls.
Adults expressing tankGD892 under the control of Scer\GAL4elav-C155 show increased resistance to ethanol sedation compared to controls. The internal ethanol concentration of these flies after exposure to 47% ethanol is not significantly different from wild type.
Males and females expressing tankGD892 under the control of Scer\GAL4tank-4-12 show increased resistance to ethanol sedation compared to controls.
Adults expressing tankGD892 under the control of either Scer\GAL49-30, Scer\GAL411-81, Scer\GAL412-27 or Scer\GAL412-234 show increased resistance to ethanol sedation compared to controls.
Expression of tankGD892 under the control of either Scer\GAL4ey-OK107, Scer\GAL4Ilp2.PR or Scer\GAL4c747 has no effect on ethanol sensitivity.
Adults expressing tankGD892 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Expression under the control of Scer\GAL4pnr-MD237 may result in semi-lethality, depending on the insertion line used.