FlyBase Allele Report: Dmel\Aven[GD9098]

General Information

Symbol

Dmel\Aven^GD9098

Species

D. melanogaster

Name

FlyBase ID

FBal0201481

Feature type

allele

Associated gene

Dmel\Aven

Associated Insertion(s)

Carried in Construct

P{GD9098}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD9098}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD9098

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle, with Scer\GAL4^Cg.PA

(Zou et al., 2011)

decreased cell size, with Scer\GAL4^Cg.PA

(Zou et al., 2011)

lethal | larval stage | temperature conditional, with Scer\GAL4^Act5C.PU

(Zou et al., 2011)

lethal | larval stage | temperature conditional, with Scer\GAL4^da.G32

(Zou et al., 2011)

lethal | pupal stage | temperature conditional, with Scer\GAL4^Act5C.PU

(Zou et al., 2011)

lethal | pupal stage | temperature conditional, with Scer\GAL4^da.G32

(Zou et al., 2011)

lethal - all die before end of pupal stage | heat sensitive, with Scer\GAL4^Cg.PA

(Zou et al., 2011)

lethal - all die before end of pupal stage | heat sensitive, with Scer\GAL4^fat

(Zou et al., 2011)

some die during pupal stage | heat sensitive, with Scer\GAL4^Cg.PA

(Zou et al., 2011)

some die during pupal stage | heat sensitive, with Scer\GAL4^fat

(Zou et al., 2011)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^elav.PU

(Zou et al., 2011)

viable, with Scer\GAL4^GMR.PU

(Zou et al., 2011)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^ptc-559.1

(Zou et al., 2011)

Phenotype Manifest In

adult fat body, with Scer\GAL4^Cg.PA

(Zou et al., 2011)

ovary, with Scer\GAL4^Cg.PA

(Zou et al., 2011)

Detailed Description

Statement

Reference

Expression of Aven^GD9098 driven by either Scer\GAL4^da.G32 or Scer\GAL4^Act5C.PU results in larval lethality at 29[o]C and pupal lethality at 25[o]C.

Expression of Aven^GD9098 driven by Scer\GAL4^elav.PU, Scer\GAL4^GMR.PU or Scer\GAL4^ptc-559.1 results in viable flies with no apparent phenotype.

Expression of Aven^GD9098 driven by either Scer\GAL4^Cg.PA or Scer\GAL4^fat results in pupal lethality at 29[o]C.

Female Aven^GD9098, Scer\GAL4^Cg.PA flies are viable at 25[o]C. Their overall body size is not obviously different from controls, but they appear to have smaller ovaries and increased body fat tissue mass. Fat cells from abdominal fat tissue are smaller and display highly condensed DNA morphology compared to controls. These fat cells also display a markedly elevated number of phospho-histone H3-positive cells.

(Zou et al., 2011)

Adults expressing Aven^GD9098 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

9098

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v19577

w¹¹¹⁸; P{GD9098}v19577

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

9098

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Aven^GD9098

CG15727^GD9098

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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