FlyBase Allele Report: Dmel\Cubn[GD6458]

General Information

Symbol

Dmel\Cubn^GD6458

Species

D. melanogaster

Name

FlyBase ID

FBal0203450

Feature type

allele

Associated gene

Dmel\Cubn

Associated Insertion(s)

Carried in Construct

P{GD6458}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD6458}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD6458

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of kidney disease

CEA

(Zhang et al., 2013)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Scer\GAL4^Ugt36A1.PK

(Feng et al., 2021)

chemical sensitive, with Scer\GAL4^Ugt36A1.PK

(Zhang et al., 2013)

increased speed of aging, with Scer\GAL4^Ugt36A1.PK

(Feng et al., 2021)

short lived, with Scer\GAL4^Ugt36A1.PK

(Feng et al., 2021)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

viable, with Scer\GAL4^Ugt36A1.PK

(Zhang et al., 2013)

Phenotype Manifest In

adult brain, with Scer\GAL4^Ugt36A1.PK

(Feng et al., 2021)

embryonic/larval pericardial cell, with Scer\GAL4^Ugt36A1.PK

(Zhang et al., 2013, Zhang et al., 2013)

indirect flight muscle cell, with Scer\GAL4^Ugt36A1.PK

(Feng et al., 2021)

Detailed Description

Statement

Reference

Scer\GAL4^Dot.PK-mediated expression of Cubn^GD6458 reduces the normal accumulation of ANF-RFP (expressed via Rnor\ANF^{Mhc.T:Disc\RFP}) in pericardial nephrocytes.

(Zhang et al., 2013)

Uptake of a secreted marker protein by pericardial nephrocytes is blocked in animals expressing Cubn^GD6458 under the control of Scer\GAL4^Dot.PK. The pericardial nephrocytes show ultrastructural defects compared to wild type: the number of endocytic vesicles and vacuoles are dramatically reduced, electron-dense lysosomes are nearly absent, nephrocyte diaphragms are reduced in number and lacunar structures are shortened.

Animals expressing Cubn^GD6458 under the control of Scer\GAL4^Dot.PK are viable under normal conditions, but show dramatically reduced viability compared to controls in the presence of silver nitrate.

(Zhang et al., 2013)

Expression via P{GAL4-tim.E}27 or P{cry-GAL4.Z}17 (in the presence of Dcr-2^Scer\UAS.cDa) does not significantly affect circadian rhythmicity in locomotor activity assays in constant darkness.

(Mandilaras and Missirlis, 2012)

Expression under the control of Scer\GAL4^pnr-MD237 results in a colour difference between the central Scer\GAL4^pnr-MD237 expression domain of the notum and the surrounding lateral region in 0% or 20-30% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

6458

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The block in uptake of a secreted marker protein by pericardial nephrocytes caused by expression of Cubn^GD6458 under the control of Scer\GAL4^Dot.PK is not rescued by co-expression of Amnionless^Scer\UAS.cZa.

(Zhang et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v14613