FlyBase Allele Report: Dmel\Nab2[GD11745]

General Information

Symbol

Dmel\Nab2^GD11745

Species

D. melanogaster

Name

FlyBase ID

FBal0204788

Feature type

allele

Associated gene

Dmel\Nab2

Associated Insertion(s)

Carried in Construct

P{GD11745}

Also Known As

dNab2 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD11745}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD11745

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of intellectual disability

CEA

(Kelly et al., 2016)

model of autosomal recessive intellectual developmental disorder

CEA

(Bienkowski et al., 2017, Pak et al., 2011)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of autosomal recessive intellectual developmental disorder

is exacerbated by Fmr1^Δ113M

(Bienkowski et al., 2017)

is exacerbated by Fmr1^UAS.cWa

(Bienkowski et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight, with Scer\GAL4^elav.PU

(Pak et al., 2011)

abnormal locomotor behavior | adult stage, with Scer\GAL4^elav.PU

(Pak et al., 2011)

abnormal locomotor behavior | adult stage, with Scer\GAL4^elav-C155

(Bienkowski et al., 2017)

abnormal locomotor behavior | adult stage, with Scer\GAL4^RapGAP1-OK6

(Pak et al., 2011)

abnormal memory | adult stage, with Scer\GAL4^elav-C155

(Kelly et al., 2016)

abnormal neuroanatomy | adult stage, with Scer\GAL4^elav-C155

(Kelly et al., 2016)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

adult mushroom body alpha-lobe, with Scer\GAL4^elav-C155

(Kelly et al., 2016)

adult mushroom body beta-lobe, with Scer\GAL4^elav-C155

(Kelly et al., 2016)

ellipsoid body, with Scer\GAL4^elav-C155

(Kelly et al., 2016)

Detailed Description

Statement

Reference

Expression of Nab2^GD11745 under the control of Nab2^GD11745 leads to climbing defects in adult flies.

(Bienkowski et al., 2017)

Expression of Nab2^GD11745 (along with UAS-dicer2) driven by Scer\GAL4^elav-C155 leads to ellipsoid body defects (lack a completely closed ring structure, with an opening left on the ventral side) and mushroom body defects (similar to those seen in Nab2^ex3/Nab2^ex3 flies: thinned/absent alpha/beta-lobes, variable degrees of beta-lobe fusion) in the adult brain, compared to controls. Mushroom body defects are more frequent in males than females. Expression of Nab2^GD11745 driven by Scer\GAL4^elav-C155 does not affect suppression of courtship index (in a courtship conditioning assay), showing learning is similar to controls; however, short-term memory is defective in Scer\GAL4^elav-C155>Nab2^GD11745 flies (trained flies show similar courtship index to sham flies). Locomotor activity in Scer\GAL4^elav-C155>Nab2^GD11745 flies is normal.

(Kelly et al., 2016)

Expression of Nab2^GD11745 under the control of Scer\GAL4^elav.PU results in flight defects and in severe locomotion defects in a negative geotaxis assay.

Expression of Nab2^GD11745 under the control of Scer\GAL4^OK6 results in modest locomotion defects in a negative geotaxis assay.

Expression of Nab2^GD11745 under the control of either Scer\GAL4^Mef2.PR or Scer\GAL4^Mhc.PW does not result in locomotion defects in a negative geotaxis assay.

(Pak et al., 2011)

Adults expressing Nab2^GD11745 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

11745

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Nab2^GD11745, Scer\GAL4^elav-C155 has abnormal locomotor behavior | adult stage phenotype, enhanceable by Fmr1^Δ113M/Fmr1[+]

(Bienkowski et al., 2017)

Nab2^GD11745, Scer\GAL4^elav-C155 has abnormal locomotor behavior | adult stage phenotype, enhanceable by Fmr1^UAS.cWa, Scer\GAL4^elav-C155

(Bienkowski et al., 2017)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The locomotion deficit (decreased climbing ability) characteristic for adult flies expressing Nab2^GD11745 under the control of Scer\GAL4^elav-C155 is worsened further by combination with a single copy of Fmr1^Δ113M but also by co-expression of Fmr1^Scer\UAS.cWa (which on its own also induces climbing ability defects).

(Bienkowski et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v27487

w¹¹¹⁸; P{GD11745}v27487

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

11745

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG5720^GD11745

Nab2^GD11745

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

Open Close