The expression of barc^GD9921 under the control of Scer\GAL4^insc-Mz1407 leads to a significant increase in the number of intermediate neural progenitors, namely of immature Ase-positive, Dpn-negative intermediates, and to significant decreases in the numbers of ganglion mother cells and of Pros-positive neurons in type II neuroblast lineages, as compared to controls. This expression also leads to significant increases in the cell size of type I and type II neuroblasts, intermediate neural progenitors, and ganglion mother cells, as compared to controls. Expression under the control of either Scer\GAL4^ase.neuro or Scer\GAL4^insc-Mz1407 leads to a significant decrease in the number of neuroblasts and to the converse significant increase in the number of ganglion mother cells in type I neuroblast lineages, as compared to controls.

Individuals expressing barc^GD9921 under the control of Scer\GAL4^pnt-14-94 are viable and reach adulthood. This expression leads to adult brains exhibiting severe neuroanatomical defects in the central complex (the ellipsoid body, fan-shaped body, and nodoli are all deformed) and exhibiting a decrease in the number of type II neuroblast-derived neurons, as compared to controls.

The expression of barc^GD9921 leads to a significant decrease in the division rates of type II neuroblasts and intermediate neural progenitors (under the combined control of Scer\GAL4^wor.PA, Scer\GAL80^ase.PN) and of type I neuroblasts and ganglion mother cells (under the control of Scer\GAL4^ase.neuro alone), as shown by their shorter cell cycle time and their higher proportion of cells in G2/M compared to controls.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in an increase in the number of neuroblasts in the larval brain compared to controls.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in longer neuroblast lineages (more daughter cells per neuroblast) in the larval brain compared to controls.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in mild defects in the shape of ganglion mother cells in the larval brain.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in an increase in the average size of ganglion mother cells in the larval brain compared to controls.

Adults expressing barc^GD9921 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression under the control of Scer\GAL4^Mef2.PR results in adult/pharate lethality.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 100% of the Scer\GAL4^pnr-MD237-expressing area of the notum.