FlyBase Allele Report: Dmel\Pfdn5[GD15259]

General Information

Symbol

Dmel\Pfdn5^GD15259

Species

D. melanogaster

Name

FlyBase ID

FBal0205306

Feature type

allele

Associated gene

Dmel\Pfdn5

Associated Insertion(s)

Carried in Construct

P{GD15259}

Also Known As

pfdn5 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD15259}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD15259

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^insc-Mz1407

(Zhang et al., 2016)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

(Zhang et al., 2016)

lethal, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

embryonic/larval brain | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

type II neuroblast | increased number | third instar larval stage, with Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

(Zhang et al., 2016)

type I neuroblast | increased number | third instar larval stage, with Scer\GAL4^insc-Mz1407

(Zhang et al., 2016)

Detailed Description

Statement

Reference

Expression of Pfdn5^GD15259 under the control of either Scer\GAL4^insc-Mz1407 (with UAS-Dicer2 to enhance RNAi efficiency) or Scer\GAL4^wor.PA (together with Scer\GAL80^ase.PN) results in formation of ectopic neuroblasts (either type I or type II, respectively) in third instar larval brains.

(Zhang et al., 2016)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in an increased number and size of GFP-positive aggregates within neuroblast lineage cells in the larval brain compared to controls (these aggregates may indicate cell death or a disturbance of internal cellular structures).

(Neumüller et al., 2011)

Adults expressing CG7048^GD15259 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 40-50% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

15259

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Pfdn2^GD13510 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4^erm.PU in pins^P89 mutant background leads to dedifferentiation of intermediate neural progenitors back into neuroblasts (ectopic type II neuroblasts are observed).

(Zhang et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v29812