UASt regulatory sequences drive expression of an inverted repeat.
Third instar larvae expressing bugGD16740 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4ppk.1.9 display hyposensitive behavioral response (lengthened latency till response to 46[o]C thermal probe stimulus) in a thermal nociception assay. However, bugGD16740 RNAi expression does not affect dendrite coverage in the ddaC dorsal multidendritic neurons compared to controls.
Adults expressing CG8297GD16740 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.