UASt regulatory sequences drive expression of an inverted repeat.
pupa, with Scer\GAL4phtm.PO
Expression of Cyp6t3GD5748 under the control of Scer\GAL4phm.PO results in very large pupae, consistent with a longer feeding period. L2 prepupae are not seen.
Adults expressing Cyp6t3GD5748 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.