UASt regulatory sequences drive expression of an inverted repeat.
Expression of sNPF-RGD661 under the control of Scer\GAL41003.3 and Scer\GAL80tsh-GAL80 or Scer\GAL4ppk.PG in larvae significantly reduces nociceptive movement responses following stimulation with a von Frey filament, compared to controls.
Adults expressing sNPF-RGD661 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.