UASt regulatory sequences drive expression of an inverted repeat.
Adults expressing Pp1α-96AGD11970 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Expression under the control of Scer\GAL4Mef2.PR results in late pupal lethality.
Scer\GAL4hs.PB- or Scer\GAL4Myo31DF-NP0001-mediated expression of Pp1α-96AGD11970 results in significantly earlier death following infection with S. marcescens compared to controls.
Scer\GAL4Myo31DF-NP0001-mediated expression of Pp1α-96AGD11970 results in a marked reduction in the number of large polyploid nuclei (which signify differentiated enterocytes) and a significant increase in DNA synthesis in the gut epithelium after 5 days of infection with S. marcescens, compared to controls.
Scer\GAL4esg-NP5130-mediated expression of Pp1α-96AGD11970, controlled using Scer\GAL80ts.αTub84B, results in significantly earlier death following infection with S. marcescens compared to controls. These flies are viable in absence of infection.
Scer\GAL4esg-NP5130-mediated expression of Pp1α-96AGD11970 severely depletes mature enterocytes in the gut epithelium after 5 days of infection with S. marcescens, compared to controls.
Expression under the control of Scer\GAL4pnr-MD237 may result in lethality before the pupal stage, depending on the insertion line used.