Expression of Sb^GD473 under the control of Scer\GAL4^ptc-559.1 narrows the L3-L4 intervein region and reduces the anterior crossvein. Expression driven by Scer\GAL4^C-765 reduces the size of both the anterior and posterior compartments and appears to primarily affect proximo-distal growth. Wings are 32% shorter than controls, and many wings appear crumpled. In addition, vein bifurcations and ectopic veins, including a stretch positioned anterior to L4, are observed frequently. Expression of Sb^GD473 exclusively in the posterior compartment using Scer\GAL4^en-e16E has only mild effects. Overall wing size is unaffected and bifurcations of the posterior cross vein are observed but rarely.

Adults expressing Sb^GD473 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Males that express Sb^GD473 under the control of Scer\GAL4^Act5C have short, stubby bristles on the notum.