UASt regulatory sequences drive expression of an inverted repeat.
Expression of Taf8GD12141 RNAi under the control of Scer\GAL4Act5C.PU (using tub-Gal80[ts] to limit the RNAi expression to the adult stage) results in germline stem cell differentiation defects in adult testes.
Ectopic expression of Taf8GD12141 under the control of Scer\GAL4phm.PU leads to developmental failure at late third larval instar when the vast majority of the animals fail to form a puparium at all and the rest dies during the P1 stage.
Adults expressing Taf8GD12141 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Expression under the control of Scer\GAL4Mef2.PR results in late pupal lethality.
Expression under the control of Scer\GAL4pnr-MD237 may result in lethality, depending on the insertion line used.
Expression under the control of Scer\GAL4pnr-MD237 results in the absence of 100% of the Scer\GAL4pnr-MD237-expressing area of the notum.