FlyBase Allele Report: Dmel\Cdc16[GD12028]

General Information

Symbol

Dmel\Cdc16^GD12028

Species

D. melanogaster

Name

FlyBase ID

FBal0209236

Feature type

allele

Associated gene

Dmel\Cdc16

Associated Insertion(s)

Carried in Construct

P{GD12028}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD12028}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD12028

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

abnormal size | adult stage, with Scer\GAL4^ey.PU

(Martins et al., 2017)

increased occurrence of cell division | third instar larval stage, with Scer\GAL4^ey.PU

(Martins et al., 2017)

lethal, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

visible | adult stage, with Scer\GAL4^ey.PU

(Martins et al., 2017)

Phenotype Manifest In

eye, with Scer\GAL4^ey.PU

(Martins et al., 2017)

eye, with Scer\GAL4^GMR.PU

(Martins et al., 2017)

eye disc | third instar larval stage, with Scer\GAL4^dac-GAL4

(Martins et al., 2017)

eye disc | third instar larval stage, with Scer\GAL4^ey.PU

(Martins et al., 2017)

eye disc | third instar larval stage, with Scer\GAL4^h-1J3

(Martins et al., 2017)

eye disc morphogenetic furrow | third instar larval stage, with Scer\GAL4^dac-GAL4

(Martins et al., 2017)

eye disc morphogenetic furrow | third instar larval stage, with Scer\GAL4^ey.PU

(Martins et al., 2017)

eye disc morphogenetic furrow | third instar larval stage, with Scer\GAL4^h-1J3

(Martins et al., 2017)

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

The expression of Cdc16^GD12028 under the control of Scer\GAL4^ey.PU leads to eye defects, including a mild decrease in eye size, severe eye morphology and polarity defects and the frequent formation of ectopic structures within the eye field that resemble naked head cuticles or antennae (indicative of a complete loss or a conversion of cell fate), as compared to controls; in combination with Dicer-2 (for more efficient RNAi) there is a strong reduction in eye size, as compared to controls. In the third instar larval eye disc, this expression strongly inhibits morphogenetic furrow progression (particularly evident in the lateral margin regions) and retinal differentiation, and leads to a significant increase in mitotic index, without affecting the disc size, as compared to controls; when expressed at 18[o]C temperature, the morphogenetic furrow progression is inhibited in the lateral margins without affecting the mitotic index, as compared to controls.

The expression of Cdc16^GD12028 under the control of Scer\GAL4^dac-GAL4 leads to morphogenetic furrow progression defects and to a small but significant reduction of the differentiated area in the third instar larval eye disc, as compared to controls. Expression under the control of Scer\GAL4^h-1J3 leads to a delay in morphogenetic furrow progression in the lateral regions of the eye disc only when in combination with Dicer-2 (for more efficient RNAi). Expression under the control of Scer\GAL4^GMR.PU leads to a rough eye phenotype only under high temperature conditions (29[o]C) or when in combination with Dicer-2 (for more efficient RNAi), as compared to controls.

(Martins et al., 2017)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in loss of neuroblasts in the larval brain associated with either increased or decreased size of the remaining neuroblasts (depending on the insertion line used).

Expression under the control of Scer\GAL4¹⁴⁰⁷ may result in mild defects in the shape of neuroblasts in the larval brain (depending on the insertion line used).

(Neumüller et al., 2011)

Adults expressing Cdc16^GD12028 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 60-70% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 70-80% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12028

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Cdc16^GD12028, Scer\GAL4^ey.PU has abnormal size | adult stage phenotype, enhanceable by Df(3L)H99

(Martins et al., 2017)

Cdc16^GD12028, Scer\GAL4^ey.PU has visible | adult stage phenotype, enhanceable by Nek2^UAS.cMa.GFP, Scer\GAL4^ey.PU

(Martins et al., 2017)

Cdc16^GD12028, Scer\GAL4^ey.PU has abnormal size | adult stage phenotype, enhanceable by Nek2^Ubi-p63E.GFP

(Martins et al., 2017)

Cdc16^GD12028, Scer\GAL4^ey.PU has visible | adult stage phenotype, enhanceable by Nek2^Ubi-p63E.GFP

(Martins et al., 2017)

Cdc16^GD12028, Scer\GAL4^ey.PU has abnormal size | adult stage phenotype, enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^ey.PU

(Martins et al., 2017)

Cdc16^GD12028, Scer\GAL4^ey.PU has visible | adult stage phenotype, enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^ey.PU

(Martins et al., 2017)

Cdc16^GD12028, Scer\GAL4^ey.PU has visible | adult stage phenotype, enhanceable by Df(3L)H99

(Martins et al., 2017)

Cdc16^GD12028, Scer\GAL4^ey.PU has abnormal size | adult stage phenotype, enhanceable by Nek2^UAS.ORF, Scer\GAL4^ey.PU

(Martins et al., 2017)

Cdc16^GD12028, Scer\GAL4^ey.PU has visible | adult stage phenotype, enhanceable by Nek2^UAS.ORF, Scer\GAL4^ey.PU

(Martins et al., 2017)

Cdc16^GD12028, Scer\GAL4^ey.PU has abnormal size | adult stage phenotype, enhanceable by Nek2^UAS.cMa.GFP, Scer\GAL4^ey.PU

(Martins et al., 2017)

NOT Enhanced by

Statement

Reference

Cdc16^GD12028, Scer\GAL4^ey.PU has increased occurrence of cell division | third instar larval stage phenotype, non-enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^ey.PU

(Martins et al., 2017)

Suppressed by

Statement

Reference

Cdc16^GD12028, Scer\GAL4^ey.PU has abnormal size | adult stage phenotype, suppressible by Nek2^KK100496, Scer\GAL4^ey.PU

(Martins et al., 2017)

Cdc16^GD12028, Scer\GAL4^ey.PU has abnormal size | adult stage phenotype, suppressible by Nek2^HM05088, Scer\GAL4^ey.PU

(Martins et al., 2017)

Cdc16^GD12028, Scer\GAL4^ey.PU has visible | adult stage phenotype, suppressible by Nek2^KK100496, Scer\GAL4^ey.PU

(Martins et al., 2017)

Cdc16^GD12028, Scer\GAL4^ey.PU has visible | adult stage phenotype, suppressible by Nek2^HM05088, Scer\GAL4^ey.PU

(Martins et al., 2017)

NOT suppressed by

Statement

Reference

Cdc16^GD12028, Scer\GAL4^ey.PU has increased occurrence of cell division | third instar larval stage phenotype, non-suppressible by Nek2^KK100496, Scer\GAL4^ey.PU

(Martins et al., 2017)

NOT Suppressor of

Statement

Reference

Cdc16^GD12028, Scer\GAL4^ey.PU is a non-suppressor of abnormal size | adult stage phenotype of CycA^GD8653, Scer\GAL4^ey.PU

(Martins et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

Cdc16^GD12028, Scer\GAL4^ey.PU has eye phenotype, enhanceable by Df(3L)H99

(Martins et al., 2017)

Cdc16^GD12028, Scer\GAL4^ey.PU has eye phenotype, enhanceable by Nek2^Ubi-p63E.GFP

(Martins et al., 2017)

Cdc16^GD12028, Scer\GAL4^ey.PU has eye phenotype, enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^ey.PU

(Martins et al., 2017)

Cdc16^GD12028, Scer\GAL4^ey.PU has eye disc | third instar larval stage phenotype, enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^ey.PU

(Martins et al., 2017)

Cdc16^GD12028, Scer\GAL4^ey.PU has eye disc morphogenetic furrow | third instar larval stage phenotype, enhanceable by Nek2^HM05088, Scer\GAL4^ey.PU

(Martins et al., 2017)

Cdc16^GD12028, Scer\GAL4^ey.PU has eye disc | third instar larval stage phenotype, enhanceable by Nek2^HM05088, Scer\GAL4^ey.PU

(Martins et al., 2017)

Cdc16^GD12028, Scer\GAL4^ey.PU has eye phenotype, enhanceable by Nek2^UAS.ORF, Scer\GAL4^ey.PU

(Martins et al., 2017)

Cdc16^GD12028, Scer\GAL4^ey.PU has eye phenotype, enhanceable by Nek2^UAS.cMa.GFP, Scer\GAL4^ey.PU

(Martins et al., 2017)

Suppressed by

Statement

Reference

Cdc16^GD12028, Scer\GAL4^dac-GAL4 has eye disc morphogenetic furrow | third instar larval stage phenotype, suppressible by Nek2^KK100496/Scer\GAL4^ey.PU

(Martins et al., 2017)

Cdc16^GD12028, Scer\GAL4^ey.PU has eye phenotype, suppressible by Nek2^KK100496, Scer\GAL4^ey.PU

(Martins et al., 2017)

Cdc16^GD12028, Scer\GAL4^ey.PU has eye disc | third instar larval stage phenotype, suppressible by Nek2^KK100496, Scer\GAL4^ey.PU

(Martins et al., 2017)

Cdc16^GD12028, Scer\GAL4^ey.PU has eye disc morphogenetic furrow | third instar larval stage phenotype, suppressible by Nek2^KK100496, Scer\GAL4^ey.PU

(Martins et al., 2017)

Cdc16^GD12028, Scer\GAL4^ey.PU has eye phenotype, suppressible by Nek2^HM05088, Scer\GAL4^ey.PU

(Martins et al., 2017)

Cdc16^GD12028, Scer\GAL4^ey.PU has eye disc | third instar larval stage phenotype, suppressible by Nek2^HM05088, Scer\GAL4^ey.PU

(Martins et al., 2017)

Cdc16^GD12028, Scer\GAL4^ey.PU has eye disc morphogenetic furrow | third instar larval stage phenotype, suppressible by Nek2^HM05088, Scer\GAL4^ey.PU

(Martins et al., 2017)

Cdc16^GD12028, Scer\GAL4^dac-GAL4 has eye disc | third instar larval stage phenotype, suppressible by Nek2^KK100496/Scer\GAL4^ey.PU

(Martins et al., 2017)

NOT Suppressor of

Statement

Reference

Cdc16^GD12028, Scer\GAL4^ey.PU is a non-suppressor of eye phenotype of CycA^GD8653, Scer\GAL4^ey.PU

(Martins et al., 2017)

Other

Statement

Reference

Cdc16^GD12028, Nek2^UAS.ORF, Scer\GAL4^dpp.PU has eye disc | third instar larval stage phenotype

(Martins et al., 2017)

Cdc16^GD12028, Nek2^UAS.ORF, Scer\GAL4^dpp.PU has eye disc morphogenetic furrow | third instar larval stage phenotype

(Martins et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Df(3L)H99 enhances the small eyes induced by the expression of Cdc16^GD12028 under the control of Scer\GAL4^ey.PU.

The adult eye defects (decreased eye size, and morphology and polarity defects) and most third instar larval eye disc defects (i.e. the morphogenetic furrow progression and retinal differentiation defects, but not the increased mitotic index) induced by the expression of Cdc16^GD12028 under the control of Scer\GAL4^ey.PU are fully suppressed by the co-expression of either Nek2^KK100496 or Nek2^HM05088. Conversely, the adult eye morphology defects and the third instar larval eye disc morphogenetic furrow progression and retinal differentiation defects are enhanced by the co-expression of Nek2^Scer\UAS.ORF, leading to a severe expansion of the head case; the adult eye morphology defects are also enhanced by the co-expression of either Nek2^UAS.cMa.GFP or Nek2^Ubi-p63E.GFP.

The third instar larval eye disc morphogenetic furrow progression and retinal differentiation impairment induced by the expression of Cdc16^GD12028 under the control of Scer\GAL4^dac-GAL4 is suppressed by the co-expression of Nek2^KK100496.

The co-expression of Cdc16^GD12028 and Nek2^Scer\UAS.ORF under the control of Scer\GAL4^dpp.PU leads to a delay in third instar larval eye disc morphogenetic furrow progression, as compared to controls.

The co-expression of Cdc16^GD12028 does not suppresses the small eyes induced by the expression of CycA^GD8653 under the control of Scer\GAL4^ey.PU.

(Martins et al., 2017)

Xenogenetic Interactions

Statement

Reference

The retinal differentiation inhibition, but not the increased mitotic index, resulting from the expression of Cdc16^GD12028 under the control of Scer\GAL4^ey.PU is enhanced by the co-expression of BacA\p35^Scer\UAS.cHa, resulting in undifferentiated masses in the adult eye field.

(Martins et al., 2017)

Complementation and Rescue Data

Rescued by

Cdc16^GD12028 is rescued by Cdc16^{Ubi-p63E.mGFP6}/Scer\GAL4^ey.PU

(Martins et al., 2017)

Comments

The third instar larval eye disc differentiation impairment and adult eye morphology defects induced by the expression of Cdc16^GD12028 under the control of Scer\GAL4^ey.PU are rescued by the co-expression of Cdc16^{Ubi-p63E.T:Avic\GFP-m6}.

(Martins et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v44869

w¹¹¹⁸; P{GD12028}v44869

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 2 )

Linkouts