Significant differences in local cell contraction rates are observed between wild-type and d^GD4041-expressing tissues within the region displaying polarised d expression in the future scutellum. The orientation of significant differences are aligned with the local orientations of d anisotropy distribution. The differences in contraction rates between wild-type and d^GD4041-expressing tissues is mainly associated with a decrease in the contribution of cell rearrangements to tissue contractions in the region of d polarisation and, to a lesser extent, to cell shape changes.

Pupae expressing d^GD4041 under the control of Scer\GAL4^unspecified manifest defects in the shape of the adult scutellum.

Flies expressing d^GD4041 under the control of either Scer\GAL4^Abd-B-LDN or Scer\GAL4^tsh-md621 show normal rotation of the male genitalia.

Adults expressing d^GD4041 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 50-60% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 0% or 40-50% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 may result in mild thorax closure defects, depending on the insertion line used.