UASt regulatory sequences drive expression of an inverted repeat.
Expression of eghGD3206 under the control of either Scer\GAL4ap-md544 or Scer\GAL4elav-C155 in males (using the temperature sensitive Scer\GAL80ts.αTub84B allele to restrict expression) results in the males having a significantly reduced courtship index towards wild-type virgin females at 29[o]C (restrictive temperature for Scer\GAL80ts.αTub84B) compared to at 20[o]C (permissive temperature for Scer\GAL80ts.αTub84B).
Adults expressing eghGD3206 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.