UASt regulatory sequences drive expression of an inverted repeat.
Adult males expressing kirreGD2595 under the control of Scer\GAL4C855a show no defects in movement and are fertile. A scattered distribution of single muscle filaments are seen overlying the regular muscle sheath of the testis, in contrast to wild type.
Adults expressing kirreGD2595 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
The numbers of nephrocyte diaphragms in the garland cell nephrocytes of second instar larvae expressing kirreGD2595 under the control of Scer\GAL4sns.GCN is reduced compared to controls, although the NDs that do form appear normal by TEM analysis.
The surface of the garland cell nephrocytes of third instar larvae expressing kirreGD2595 under the control of Scer\GAL4sns.GCN is more uniform than that of control cells when analysed by SEM.
Garland cell nephrocytes from second instar larvae expressing kirreGD2595 under the control of Scer\GAL4sns.GCN show efficient uptake of dextran 10kD after co-incubation with both Cascade Blue dextran 10kD and fluorescein dextran 500kD for 5 minutes. However, these cells show a very poor uptake of dextran 500kD under these conditions compared to controls.