FlyBase Allele Report: Dmel\p24-1[GD3321]

General Information

Symbol

Dmel\p24-1^GD3321

Species

D. melanogaster

Name

FlyBase ID

FBal0210282

Feature type

allele

Associated gene

Dmel\p24-1

Associated Insertion(s)

Carried in Construct

P{GD3321}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD3321}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD3321

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

female semi-sterile, with Scer\GAL4^Act5C.PI

(Saleem et al., 2012)

lethal, with Scer\GAL4^Act5C.PI

(Saleem et al., 2012)

lethal, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

lethal, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

male sterile, with Scer\GAL4^Act5C.PI, Scer\GAL80^ts.αTub84B

(Saleem et al., 2012)

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Act5C.PI

(Saleem et al., 2012)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

sarcomere, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

wing margin, with Scer\GAL4^en.PU

(Buechling et al., 2011)

Z disc, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

Detailed Description

Statement

Reference

Flies expressing p24-1^GD3321 during adulthood under the control of Scer\GAL4^Act5C.PI and Scer\GAL80^ts.αTub84B are viable but female flies lay fewer egg per day compared to controls. Males are completely infertile.

(Saleem et al., 2012)

Scer\GAL4^en.PU-mediated expression of p24-1^GD3321 results in loss of posterior wing margin.

(Buechling et al., 2011)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in loss of neuroblasts in the larval brain associated with decreased size of the remaining neuroblasts.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in a reduction in the average size of neuroblasts together with shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

(Neumüller et al., 2011)

Adults expressing p24-1^GD3321 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in early larval lethality.

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal larval body wall muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in sarcomeres with reduced Z-lines in the larval body wall muscles.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in lethality before the pupal stage.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

3321

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v12196

w¹¹¹⁸; P{GD3321}v12196

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 2 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

3321

Neuroblasts Screen Database (Knoblich Lab) - A database for RNAi phenotypes in larval neural stem cells

3321

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

p24-1^GD3321

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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