FB2024_03 , released June 25, 2024
Allele: Dmel\Dscam14.3-6.36-9.25-17.1
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General Information
Symbol
Dmel\Dscam14.3-6.36-9.25-17.1
Species
D. melanogaster
Name
FlyBase ID
FBal0213029
Feature type
allele
Associated gene
Dmel\Dscam1
Associated Insertion(s)
Carried in Construct
P{Dscam1.4.3-6.36-9.25-17.1}
Key Links
Transgenic product class
wild_type
(Wang et al., 2004)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Wang et al., 2004)
Mutagen
in vitro construct
(Wang et al., 2004)
Progenitor genotype
Carried in construct
P{Dscam1.4.3-6.36-9.25-17.1}
Cytology
Description

A 4.5kb Dscam promoter fragment from -261 to +4309 bp (this fragment includes the 5' untranslated sequences of exon 1 and up to the exon 2 ATG codon) is fused to a Dscam cDNA that encodes exons 2-18 followed by a genomic fragment encoding Dscam exons 18-24. The cDNA fragment contains the alternative exons 4.3, 6.36, 9.25 and 17.1.

(Wang et al., 2004)
Allele components
Component
Use(s)
Regulatory region(s)
Dscam1
Encoded product / tool
Dscam1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies carrying Dscam4.3-6.36-9.25-17.1 in a wild-type background show no defects in the morphogenesis of mushroom body α/β neurons.

      (Wang et al., 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Dscam14.3-6.36-9.25-17.1/Scer\GAL4221 rescues Dscam121/Dscam123

      (Matthews et al., 2007)

      Dscam14.3-6.36-9.25-17.1 rescues Dscam118

      (Shi et al., 2007)
      Fails to rescue

      Dscam14.3-6.36-9.25-17.1 fails to rescue Dscam118

      (Shi et al., 2007)

      Dscam14.3-6.36-9.25-17.1 fails to rescue Dscam118/Dscam1B17-1

      (Wang et al., 2004)

      Dscam14.3-6.36-9.25-17.1 fails to rescue Dscam118

      (Wang et al., 2004)
      Comments

      The self-avoidance defects (dendrite crossings) seen in Dscam21/Dscam23 class I da neurons are rescued by expression of Dscam4.3-6.36-9.25-17.1.

      (Matthews et al., 2007)

      Dscam4.3-6.36-9.25-17.1 fully rescues the projection neuron dendrite defects, but has no effect on the reduced number of calyx boutons seen in Dscam18 clones.

      (Shi et al., 2007)

      Dscam4.3-6.36-9.25-17.1 shows only minimal rescue of the axon branching defects seen in single cell clones of mushroom body α/β neurons that are homozygous for Dscam18; 28% of clones show lack of segregation of sister branches and 34% show generation of additional branches at the bifurcation points (compared to 35% and 42% respectively in the absence of Dscam4.3-6.36-9.25-17.1).

      Dscam4.3-6.36-9.25-17.1 fails to rescue the early larval lethality of Dscam18/DscamB17-1 animals.

      Co-expression of both Dscam4.3-6.36-9.25-17.1 and Dscam4.3-6.36-9.25-17.2 partially rescues the early larval lethality of Dscam18/DscamB17-1 animals, such that some some animals survive to the adult stage. These adults are vegetative after eclosion and need special care for survival (some of these flies can survive for 3 days within wet chambers on grape juice agar plates). The rescued adults show multiple signs of poor brain development; all major brain compartments (including the supraesophageal ganglion, the subesophageal ganglion and the optic lobes) appear rudimentary and the paired brain lobes are incompletely fused. Loss of dorsal or medial mushroom body lobes is seen and the brains lack ellipsoid body-like structures.

      (Wang et al., 2004)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Dscam14.3-6.36-9.25-17.1
      Dscam4.3-6.36-9.25-17.1
      Name Synonyms
      Secondary FlyBase IDs
        References (3)