FlyBase Allele Report: Dmel\rumi[Δ26]

General Information

Symbol

Dmel\rumi^Δ26

Species

D. melanogaster

Name

FlyBase ID

FBal0216685

Feature type

allele

Associated gene

Dmel\rumi

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Acar et al., 2008)

Progenitor genotype

P{EPgy2}Usp12-46^EY00249

(Acar et al., 2008)

Cytology

Description

Imprecise excision of the P{EPgy2}CG7023^EY00249 insertion, resulting in a deletion that removes most of the rumi open reading frame.

(Acar et al., 2008)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal eclosion

(Leonardi et al., 2011)

abnormal neuroanatomy

(Acar et al., 2008)

lethal | heat sensitive

(Leonardi et al., 2011)

partially lethal - majority die | pharate adult stage

(Leonardi et al., 2011)

short lived

(Leonardi et al., 2011)

visible | recessive

(Leonardi et al., 2011)

Phenotype Manifest In

Detailed Description

Statement

Reference

1-day old homozygous adults raised at 18[o]C (in ambient light) show a 100% penetrant rhabdomere attachment phenotype, with 2 or more rhabdomeres in each ommatidium being attached to each other. The severity of the phenotype is similar in 15 and 40 day old mutant flies.

At 65% pupal development, the area of the inter-rhabdomeral space (IRS) in mutant ommatidia is 59% smaller than in controls, while in adults the IRS of mutant ommatidia appears similar to that of controls.

(Haltom et al., 2014)

At 25 [o]C, some homozygous rumi^Δ26 mutant animals reach adulthood but display very severe loss of bristles on the thorax and shortening of the legs. These flies die on the food after eclosion or trapped in the pupal case during eclosion, presumably owing to leg defects. As the temperature is decreased to room temperature (21-23 [o]C), approximately three quarter of the rumi^Δ26 mutants exhibit an intermediate loss of microchaetae. At room temperature, the legs are not short, but subtle leg joint defects are commonly observed. When raised at 18 [o]C, most homozygous rumi^Δ26 animals show loss of small patches of microchaetae in anterior parts of the notum and slight increase in the density of microchaetae. No shortening of the legs is observed at 18 [o]C, although most animals show minor defects in leg joints.

Homozygous rumi^Δ26 mutant animals fail to reach the pharate adult stage when cultured at temperatures above 25 [o]C.

(Leonardi et al., 2011)

rumi^Δ26 mitotic clones raised at 30[o]C exhibit the loss of thoracic sensory bristles and margin loss and vein expansion in the wing.

(Takeuchi et al., 2011)

rumi^Δ26 embryos lacking both maternal and zygotic rumi function raised at 28^oC have a neurogenic phenotype.

(Acar et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

rumi^Δ26 has visible | recessive phenotype, suppressible by N^gt-4.5

(Leonardi et al., 2011)

rumi^Δ26 has visible | recessive phenotype, suppressible by N^gt-24-35

(Leonardi et al., 2011)

rumi^Δ26 has visible | recessive phenotype, suppressible by N^gt-16-20

(Leonardi et al., 2011)

rumi^Δ26 has visible | recessive phenotype, suppressible by N^gt-16-35

(Leonardi et al., 2011)

rumi^Δ26 has visible | recessive phenotype, suppressible by N^gt-12.14.15

(Leonardi et al., 2011)

rumi^Δ26 has visible | recessive phenotype, suppressible by N^gt-12

(Leonardi et al., 2011)

rumi^Δ26 has visible | recessive phenotype, suppressible by N^gt-10.13-15

(Leonardi et al., 2011)

rumi^Δ26 has visible | recessive phenotype, suppressible | partially by N^{gt-10.12.14.15}

(Leonardi et al., 2011)

rumi^Δ26 has visible | recessive phenotype, suppressible | partially by N^gt-10-15

(Leonardi et al., 2011)

rumi^Δ26 has visible | recessive phenotype, suppressible | partially by N^gt-10-20

(Leonardi et al., 2011)

rumi^Δ26 has visible | recessive phenotype, suppressible | partially by N^gt-10-35

(Leonardi et al., 2011)

rumi^Δ26 has visible | recessive phenotype, suppressible | partially by N^gt-4-35

(Leonardi et al., 2011)

rumi^Δ26 has lethal | heat sensitive phenotype, suppressible by N^gt-4.5

(Leonardi et al., 2011)

rumi^Δ26 has lethal | heat sensitive phenotype, suppressible by N^gt-24-35

(Leonardi et al., 2011)

rumi^Δ26 has lethal | heat sensitive phenotype, suppressible by N^gt-16-20

(Leonardi et al., 2011)

rumi^Δ26 has lethal | heat sensitive phenotype, suppressible by N^gt-16-35

(Leonardi et al., 2011)

rumi^Δ26 has lethal | heat sensitive phenotype, suppressible by N^gt-12.14.15

(Leonardi et al., 2011)

rumi^Δ26 has lethal | heat sensitive phenotype, suppressible by N^gt-12

(Leonardi et al., 2011)

rumi^Δ26 has lethal | heat sensitive phenotype, suppressible by N^gt-10.13-15

(Leonardi et al., 2011)

rumi^Δ26 has lethal | heat sensitive phenotype, suppressible by N^{gt-10.12.14.15}

(Leonardi et al., 2011)

rumi^Δ26 has lethal | heat sensitive phenotype, suppressible by N^gt-10-15

(Leonardi et al., 2011)

NOT suppressed by

Statement

Reference

rumi^Δ26 has lethal | heat sensitive phenotype, non-suppressible by N^gt-10-20

(Leonardi et al., 2011)

rumi^Δ26 has lethal | heat sensitive phenotype, non-suppressible by N^gt-10-35

(Leonardi et al., 2011)

rumi^Δ26 has lethal | heat sensitive phenotype, non-suppressible by N^gt-4-35

(Leonardi et al., 2011)

Suppressor of

Statement

Reference

rumi^Δ26 is a suppressor of abnormal size phenotype of N^{ΔLNR.lexA.UAS}, Scer\GAL4^Act.PU

(Leonardi et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

rumi^Δ26 has rhabdomere phenotype, enhanceable by eys⁷³⁴/eys[+]

(Haltom et al., 2014)

rumi^Δ26 has rhabdomere phenotype, enhanceable by Hsc70-3^G0102/Hsc70-3[+]

(Haltom et al., 2014)

Suppressed by

Statement

Reference

rumi^Δ26 has notal microchaeta phenotype, suppressible by N^gt-4.5

(Leonardi et al., 2011)

rumi^Δ26 has notal microchaeta phenotype, suppressible by N^gt-24-35

(Leonardi et al., 2011)

rumi^Δ26 has notal microchaeta phenotype, suppressible by N^gt-16-20

(Leonardi et al., 2011)

rumi^Δ26 has notal microchaeta phenotype, suppressible by N^gt-16-35

(Leonardi et al., 2011)

rumi^Δ26 has notal microchaeta phenotype, suppressible by N^gt-12.14.15

(Leonardi et al., 2011)

rumi^Δ26 has notal microchaeta phenotype, suppressible by N^gt-12

(Leonardi et al., 2011)

rumi^Δ26 has notal microchaeta phenotype, suppressible by N^gt-10.13-15

(Leonardi et al., 2011)

rumi^Δ26 has notal microchaeta phenotype, suppressible | partially by N^{gt-10.12.14.15}

(Leonardi et al., 2011)

rumi^Δ26 has notal microchaeta phenotype, suppressible | partially by N^gt-10-15

(Leonardi et al., 2011)

rumi^Δ26 has notal microchaeta phenotype, suppressible | partially by N^gt-10-20

(Leonardi et al., 2011)

rumi^Δ26 has notal microchaeta phenotype, suppressible | partially by N^gt-10-35

(Leonardi et al., 2011)

rumi^Δ26 has notal microchaeta phenotype, suppressible | partially by N^gt-4-35

(Leonardi et al., 2011)

rumi^Δ26 has leg phenotype, suppressible | partially by N^gt-4-35

(Leonardi et al., 2011)

rumi^Δ26 has leg joint phenotype, suppressible | partially by N^gt-4-35

(Leonardi et al., 2011)

rumi^Δ26 has mechanosensory chaeta | somatic clone | heat sensitive phenotype, suppressible by Hsap\POGLUT1^UAS.Tag:FLAG/Scer\GAL4^αTub84B.PL

(Takeuchi et al., 2011)

rumi^Δ26 has wing margin | somatic clone | heat sensitive phenotype, suppressible by Hsap\POGLUT1^UAS.Tag:FLAG/Scer\GAL4^c684

(Takeuchi et al., 2011)

rumi^Δ26 has wing vein | somatic clone | heat sensitive phenotype, suppressible by Hsap\POGLUT1^UAS.Tag:FLAG/Scer\GAL4^c684

(Takeuchi et al., 2011)

NOT suppressed by

Statement

Reference

rumi^Δ26 has mechanosensory chaeta | somatic clone | heat sensitive phenotype, non-suppressible by Hsap\POGLUT2^UAS.Tag:FLAG

(Takeuchi et al., 2011)

rumi^Δ26 has wing margin | somatic clone | heat sensitive phenotype, non-suppressible by Scer\GAL4^c684/Hsap\POGLUT2^UAS.Tag:FLAG

(Takeuchi et al., 2011)

rumi^Δ26 has wing vein | somatic clone | heat sensitive phenotype, non-suppressible by Scer\GAL4^c684/Hsap\POGLUT2^UAS.Tag:FLAG

(Takeuchi et al., 2011)

rumi^Δ26 has mechanosensory chaeta | somatic clone | heat sensitive phenotype, non-suppressible by Scer\GAL4^αTub84B.PL/Hsap\POGLUT2^UAS.Tag:FLAG

(Takeuchi et al., 2011)

rumi^Δ26 has wing margin | somatic clone | heat sensitive phenotype, non-suppressible by Hsap\POGLUT3^UAS.Tag:FLAG/Scer\GAL4^c684

(Takeuchi et al., 2011)

rumi^Δ26 has wing vein | somatic clone | heat sensitive phenotype, non-suppressible by Hsap\POGLUT3^UAS.Tag:FLAG/Scer\GAL4^c684

(Takeuchi et al., 2011)

Suppressor of

Statement

Reference

rumi^Δ26 is a suppressor of wing disc | somatic clone | third instar larval stage phenotype of N^{ΔLNR.lexA.UAS}, Scer\GAL4^Act.PU

(Leonardi et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

eys⁷³⁴/+ strongly enhances the rhabdomere attachment phenotype of rumi^Δ26 homozygotes raised at 18[o]C: multiple rhabdomeres collapse into one another in each ommatidium and there is a dramatic decrease in the size of the inter-rhabdomeral space (although small pockets of it are still present in each ommatidium).

Hsc70-3^G0102/+ enhances the rhabdomere attachment phenotype of rumi^Δ26 homozygotes raised at 18[o]C (the average number of separate rhabdomeres is reduced). Hsc70-3^G0102/+ ; rumi^Δ26/+ double heterozygotes raised at 18[o]C do not show a rhabdomere attachment phenotype.

(Haltom et al., 2014)

Homozygous rumi^Δ26 mutant animals also carrying N^gt-10-20 fail to reach the pharate adult stage when cultured at temperatures above 25 [o]C.

Homozygous rumi^Δ26 mutant animals also carrying N^gt-10-35 fail to reach the pharate adult stage when cultured at temperatures above 25 [o]C.

Homozygous rumi^Δ26 mutant animals also carrying N^gt-4-35 fail to reach the pharate adult stage when cultured at temperatures above 25 [o]C. Mutants cultured at room temperature (21-23 [o]C), similarly to rumi^Δ26 single mutants, commonly exhibit mild defects in leg joints. At 18 [o]C, the mutants show a mild loss of microchaetae and normal legs.

rumi^Δ26 fully suppresses the N^{Scer\UAS.ΔLNR.T:Ecol\lexA} wing imaginal disc phenotypes.

(Leonardi et al., 2011)

Xenogenetic Interactions

Statement

Reference

Overexpression of Hsap\POGLUT1^{Scer\UAS.T:Zzzz\FLAG} suppresses the sensory organ and wing phenotypes of rumi^Δ26 mitotic clones raised at 30[o]C.

Overexpression of Hsap\KDELC1^{Scer\UAS.T:Zzzz\FLAG} or Hsap\KDELC2^{Scer\UAS.T:Zzzz\FLAG} fails to suppress the sensory organ and wing phenotypes of rumi^Δ26 mitotic clones raised at 30[o]C.

(Takeuchi et al., 2011)

Complementation and Rescue Data

Rescued by

rumi^Δ26 is rescued by rumi^gt.Tag:FLAG

(Haltom et al., 2014)

rumi^Δ26 is rescued by rumi^UAS.cAa/Scer\GAL4^unspecified

(Acar et al., 2008)

Comments

rumi^{gt.T:Zzzz\FLAG} rescues the rhabdomere attachment phenotype seen in rumi^Δ26 flies raised at 18[o]C.

(Haltom et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

rumi^Δ26

(Haltom et al., 2014, Fernandez-Valdivia et al., 2011, Leonardi et al., 2011, Takeuchi et al., 2011, Acar et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (5)

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