FlyBase Allele Report: Dmel\grp[fs1]

General Information

Symbol

Dmel\grp^fs1

Species

D. melanogaster

Name

FlyBase ID

FBal0216722

Feature type

allele

Associated gene

Dmel\grp

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

The grp coding region is expressed from both GMR and Scer\UAS regulatory sequences.

(Brodsky et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell cycle | third instar larval stage

(Brodsky et al., 2004)

chemical sensitive

(Lee et al., 2012)

decreased cell death | third instar larval stage

(Brodsky et al., 2004)

lethal - all die during embryonic stage | non-rescuable maternal effect

(Takada et al., 2007)

some die during embryonic stage | non-rescuable maternal effect

(Takada et al., 2007)

Phenotype Manifest In

wing disc | third instar larval stage

(Brodsky et al., 2004)

Detailed Description

Statement

Reference

Homozygous larvae are sensitive to low levels of hydroxyurea in a survival assay.

(Lee et al., 2012)

grp^fs1 has no effect on the apoptotic response seen in eye discs containing dicentric chromosomes (generated using Scer\FLP1^hs.PP-induced recombination in chromosomes containing inverted Scer\FRT sites). This effect is seen at 12-14 hours after heat shock of DcYy⁺.

(Titen and Golic, 2008)

Embryos derived from homozygous females fail to undergo cellularisation and nuclei are either lost from the surface or fuse to form aggregates. The mutant embryos exit mitosis 13 and immediately continue through additional cleavage cycles (in wild-type embryos, the cell cycle pauses after mitosis 13 and cellularisation occurs).

The mutant embryos do not undergo gastrulation.

(Takada et al., 2007)

The wing discs of grp^fs1 mutant third instar larvae exhibit similar levels of apoptosis to controls in response to X-ray induced irradiation. As in controls, very little apoptosis is seen in the absence of irradiation. grp^fs1 mutant larvae exhibit a reduction in irradiation induced cell cycle arrest.

(Brodsky et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

grp^fs1 has lethal | non-rescuable maternal effect | embryonic stage phenotype, non-suppressible by lok^p6

(Takada et al., 2007)

Enhancer of

Statement

Reference

grp^fs1 is an enhancer of abnormal cell death | conditional phenotype of lok^p6

(Titen and Golic, 2008)

Phenotype Manifest In

Enhancer of

Statement

Reference

grp^fs1 is an enhancer of eye disc | conditional phenotype of lok^p6

(Titen and Golic, 2008)

grp^fs1 is an enhancer of wing disc | conditional phenotype of lok^p6

(Titen and Golic, 2008)

grp^fs1 is an enhancer of larval neuroblast | larval stage | conditional phenotype of lok^p6

(Titen and Golic, 2008)

Additional Comments

Genetic Interactions

Statement

Reference

The developmental delay and arrest at cycle 3 characteristic for embryos from ddbt^Z4344 fathers is not affected in embryos from ddbt^Z4344 fathers and grp^fs1 mothers.

(Yamaki et al., 2016)

grp^fs1 partially suppresses the residual apoptotic response seen in lok^p6 mutants containing dicentric chromosomes (generated using Scer\FLP1^hs.PP-induced recombination in chromosomes containing inverted Scer\FRT sites). Eye disc apoptosis is abolished 12-14 hours after heat shock of DcYy⁺, P{FRT(w^hs)}(8F)105 or P{inv.FRT}Dc3-FrTr1D. Wing disc apoptosis is also suppressed in grp^fs1 lok^p6 mutants at 12-24 hours after heat shock of DcYy⁺, P{FRT(w^hs)}(8F)105 or P{inv.FRT}Dc3-FrTr1D. At 24 hours after heat shock wing disc apoptosis is suppressed in double mutant cells containing Y chromosome dicentrics, but apoptosis is still seen when dicentric chromosomes are generated on the X or autosome.

Expression of grp^GUS.cBa partially suppresses the apoptosis seen in lok^p6 grp^fs1 double mutant cells containing dicentric chromosomes (generated using Scer\FLP1^hs.PP-induced recombination in chromosomes containing inverted Scer\FRT sites). This effect is seen at 12-14 hours after heat shock of DcYy⁺.

(Titen and Golic, 2008)

A significant number of embryos derived from grp^fs1 lok^p6 double mutant females have a uniform monolayer of cortical nuclei surrounded by a hexagonal actin network, indicating that they have initiated or completed cellularisation. The nuclei in the double mutant embryos are larger than in wild-type controls. The double mutant embryos consistently proceed through a fifth cortical mitotic cycle (14th cycle) before cellularisation (in wild-type embryos, cellularisation occurs after the 13th cycle). The double mutant embryos also undergo gastrulation. The cephalic furrow does not form in the double mutant embryos.

(Takada et al., 2007)

grp^fs1 lok^p6 double mutants exhibit normal telomere protection and chromosome break repair.

(Oikemus et al., 2006)

lok^p6 enhances the partial loss of cell cycle arrest seen in grp^fs1 mutant third instar larval wing discs in response to X-ray induced irradiation. No arrest is seen in the double mutant larvae.

(Brodsky et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

grp^fs1

(Yamaki et al., 2016, Iampietro et al., 2014, Lee et al., 2012, Kurzhals et al., 2011, Titen and Golic, 2008, Takada et al., 2007, Oikemus et al., 2006)

grps^fs1

(Brodsky et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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