FlyBase Allele Report: Dmel\sdt[K85]

General Information

Symbol

Dmel\sdt^K85

Species

D. melanogaster

Name

FlyBase ID

FBal0218372

Feature type

allele

Associated gene

Dmel\sdt

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Nucleotide substitution: C44683T.

(Berger et al., 2007)

The premature stop codon is in the L27N domain.

(Berger et al., 2007)

Amino acid replacement: Q?term.

(Berger et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:8,235,917..8,235,917 [+]

Nucleotide change:

C8235917T

Reported nucleotide change:

C44683T

Amino acid change:

Reported amino acid change:

Q?term

(Berger et al., 2007)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile, with sdt^sfGFP

(Perez-Mockus et al., 2017)

viable, with sdt^sfGFP

(Perez-Mockus et al., 2017)

Phenotype Manifest In

eye photoreceptor cell stalk

(Bulgakova et al., 2010)

eye photoreceptor cell stalk | somatic clone

(Bulgakova et al., 2008, Berger et al., 2007)

follicle cell | somatic clone

(Penalva and Mirouse, 2012)

Detailed Description

Statement

Reference

Homozygous follicle cell clones show multilayering of cells in 31% of cases and show a flattened cells compared to wild-type cells in 37% of cases.

(Penalva and Mirouse, 2012)

In sdt^K85/+ retinas the photoreceptor cell stalk membrane is shorter than that of wild-type.

(Bulgakova et al., 2010)

sdt^K85 photoreceptor cell MARCM clones exhibit an approximate 50% reduction in stalk membrane length.

(Bulgakova et al., 2008)

Photoreceptors in homozygous eye clones show severe defects, including a shortened stalk membrane, and they degenerate when exposed to light. This light-induced degeneration is strongly reduced if the animals are raised and maintained on vitamin A-depleted medium.

(Berger et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

sdt[+]/sdt^K85, w¹¹¹⁸ has retina | conditional phenotype, suppressible by Scer\GAL4^GMR.PF/sdt^{UAS.H.Tag:FLAG}

(Bulgakova et al., 2010)

Enhancer of

Statement

Reference

sdt[+]/sdt^K85 is an enhancer of retina | conditional phenotype of Scer\GAL4^ninaE.PT, sdt^{UAS.D.Tag:FLAG}, w¹¹¹⁸

(Bulgakova et al., 2010)

NOT Enhancer of

Statement

Reference

sdt[+]/sdt^K85 is a non-enhancer of retina | conditional phenotype of w¹¹¹⁸

(Bulgakova et al., 2010)

NOT Suppressor of

Statement

Reference

sdt[+]/sdt^K85 is a non-suppressor of retina | conditional phenotype of w¹¹¹⁸

(Bulgakova et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The light dependent retinal degeneration seen in w¹¹¹⁸ flies does not depend on sdt^K85/+. The rate of photoreceptor cell survival seen in a w¹¹¹⁸ genetic background is similar in sdt^K85/+ or +/+ flies.

Expression of sdt^{Scer\UAS.H.T:Zzzz\FLAG} in outer photoreceptor cells under the control of Scer\GAL4^ninaE.PT in sdt^K85/+ or +/+ genetic background completely blocked the light induced retinal degeneration in w¹¹¹⁸ flies. In addition, the rhabdomeres of the outer Scer\GAL4^ninaE.PT-expressing photoreceptor cells are reduced in size in comparison with control flies and flies including flies kept in the dark.

Expression of sdt^{Scer\UAS.D.T:Zzzz\FLAG} under the control of Scer\GAL4^ninaE.PT enhances the retinal degeneration phenotype in w¹¹¹⁸ eyes. When sdt^{Scer\UAS.D.T:Zzzz\FLAG} is expressed in a sdt^K85/+ background, the decrease in photoreceptor cell survival is stronger.

(Bulgakova et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

sdt^E195

(Berger et al., 2007)

sdt^K70

(Berger et al., 2007)

sdt^XN

(Berger et al., 2007)

sdt^M120

(Berger et al., 2007)

sdt^XH21

(Berger et al., 2007)

sdt^EH681

(Berger et al., 2007)

sdt^7D

(Berger et al., 2007)

sdt^XP

(Berger et al., 2007)

Partially rescued by

sdt^K85 is partially rescued by sdt^{A.UAS.Tag:FLAG}/Scer\GAL4^ninaE.PT

(Bulgakova et al., 2008)

sdt^K85 is partially rescued by sdt^{B2.UAS.Tag:FLAG}/Scer\GAL4^ninaE.PT

(Bulgakova et al., 2008)

Not rescued by

sdt^K85 is not rescued by sdt^{ΔN.UAS.Tag:FLAG}/Scer\GAL4^ninaE.PT

(Bulgakova et al., 2008)

sdt^K85 is not rescued by sdt^{ΔL27C.UAS.Tag:FLAG}/Scer\GAL4^ninaE.PT

(Bulgakova et al., 2008)

sdt^K85 is not rescued by Scer\GAL4^ninaE.PT/sdt^{ΔPDZ.UAS.Tag:FLAG}

(Bulgakova et al., 2008)

sdt^K85 is not rescued by sdt^{ΔSH3.UAS.Tag:FLAG}/Scer\GAL4^ninaE.PT

(Bulgakova et al., 2008)

sdt^K85 is not rescued by sdt^{ΔGUK.UAS.Tag:FLAG}/Scer\GAL4^ninaE.PT

(Bulgakova et al., 2008)

sdt^K85 is not rescued by sdt^{1-L27C.UAS.Tag:FLAG}/Scer\GAL4^ninaE.PT

(Bulgakova et al., 2008)

Comments

Expression of sdt^{A.Scer\UAS.T:Zzzz\FLAG} or sdt^{B2.Scer\UAS.T:Zzzz\FLAG} in sdt^K85 photoreceptor cell MARCM clones partially restores talk-membrane length to a reduction of only 30% compared with wild-type. This stalk is significantly longer than that in photoreceptor cells mutant for sdt^K85, but significantly shorter than stalk-membrane length in control photoreceptor cells.

(Bulgakova et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

99629

y¹ w^* sdt^K85 P{neoFRT}19A/FM7i, P{ActGFP}JMR3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

sdt^K85

(Wang et al., 2018, Perez-Mockus et al., 2017, Spannl et al., 2017, Koch et al., 2016, Sen et al., 2015, Penalva and Mirouse, 2012, Bulgakova et al., 2010, Krahn et al., 2010, Bulgakova et al., 2008, Berger et al., 2007)

sdt^k85

(Salis et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (11)

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