P{PZ} insertion 8bp upstream of the first untranslated exon.
41% of homozygotes survive to adulthood, with no detectable developmental delay, abnormalities or size differences.
More than half of heterozygous leg discs which are cut and then cultured in vivo for 48 hours still show asynchronous cell divisions (this period of time is sufficient for blastema formation in wild-type leg disc fragments). However, if the rgn1 fragments are cultured for 72 hours, a blastema is formed in 68% of discs.
Homozygotes have a multiple bristle phenotype.
visible multiple bristles
The formation of a regeneration blastema that is seen in the proximodistal portion of the leg disc after induction of wgAct5C.PS expression in the early third larval instar is delayed by rgn1/+; no blastema is seen in these animals 48 hours after induction of wgAct5C.PS expression, but a regeneration blastema is seen 72 hours after induction of wgAct5C.PS expression.
The frequency of adults that show transdetermination (wing structures in the adult legs) after induction of wgAct5C.PS 72 hours after egg deposition is reduced to 7% if the animals are also carrying rgn1/+.
A. Spradling.
This allele was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was discarded from the gene disruption project prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Spradling (spradling@mail1.ciwemb.edu).
Complements: l(3)0406304063.