FB2024_03 , released June 25, 2024
Insertion: Dmel\P{PZ}rgn00534
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General Information
Symbol
Dmel\P{PZ}rgn00534
Species
D. melanogaster
Name
FlyBase ID
FBti0116131
Feature type
transposable_element_insertion_site
Description
Inserted Element
P{PZ}
LINE ID

l(3)00534

Affected gene(s)
Ecol\lacZ, rgn
Viability / fertility
Associated allele(s)
Ecol\lacZ00534, rgn00534
Stock availability
None publicly available
Genomic Location
Chromosomal Location
3L ( 78C9 )
Sequence Location
3L:21,382,516..21,382,516 [+]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{PZ}.BDGP
Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
3L:21,382,516..21,382,516 [+]
(McClure et al., 2008)
3L:21,382,517..21,382,517 [+]
(Gene Disruption Project members, 2015.10.8)
Orientation

+

(Gene Disruption Project members, 2015.10.8, McClure et al., 2008)
Cytological location (computed by FlyBase)
78C9 (inferred by FlyBase from sequence location)
Cytological location (reported)
78C9_r6 (reported as inferred from sequence location)
(Gene Disruption Project members, 2015.10.8)
78D1-78D2 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Inserted Element
Construct
P{PZ}
(McClure et al., 2008)
Size (Kb)

14.545

Component Allele(s)
l(3)87Df7.2
ry+t7.2
Molecular map
Affected Gene(s)
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
wandering third instar larval stage
imaginal disc
(Meister and Braun, 1995.10)
embryonic/larval tracheal section
(Meister and Braun, 1995.10)
embryonic/larval hindgut
(Meister and Braun, 1995.10)
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Line ID

l(3)00534

(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

Possible second insertion in trbl gene at 3L:20401372 (minus strand) (release 6).

(Gene Disruption Project members, 2015.10.8)

Reported as 8 base pairs upstream of the first untranslated exon of rgn (calculated based on GB:AF145671 start).

(McClure et al., 2008)

This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).

(FlyBase, 1992-)
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
P{PZ}l(3)0053400534
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{PZ}rgn00534
P{PZ}rgn1
(FlyBase, 1992-)
P{ry+t7.2=PZ}l(3)0053400534
(FlyBase, 1992-)
l(3)00534
(Gene Disruption Project members, 2015.10.8)
Name Synonyms
Secondary FlyBase IDs
  • FBti0115993
  • FBti0000468
  • FBti0005404
References (12)