FlyBase Allele Report: Dmel\Rab11[mo]

General Information

Symbol

Dmel\Rab11^mo

Species

D. melanogaster

Name

malformed ommatidia

FlyBase ID

FBal0230368

Feature type

allele

Associated gene

Dmel\Rab11

Associated Insertion(s)

P{lacW}Rab11^mo

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Alone et al., 2005)

Progenitor genotype

Associated Insertion(s)

P{lacW}Rab11^mo

Cytology

Description

Insertion in the 5' UTR.

(Alone et al., 2005)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death

(Tiwari and Roy, 2009)

increased cell death | third instar larval stage

(Alone et al., 2005)

lethal - all die before end of larval stage (with Rab11^EP3017)

(Alone et al., 2005)

partially lethal - majority die

(Alone et al., 2005)

some die during larval stage (with Rab11^EP3017)

(Alone et al., 2005)

sterile | recessive

(Alone et al., 2005)

visible

(Alone et al., 2005)

Phenotype Manifest In

chaeta & antenna

(Alone et al., 2005)

cone cell

(Tiwari and Roy, 2009)

eye | pupal stage

(Tiwari and Roy, 2009)

eye disc

(Alone et al., 2005)

eye photoreceptor cell

(Tiwari and Roy, 2009, Alone et al., 2005)

interommatidial bristle

(Tiwari and Roy, 2009, Alone et al., 2005)

interommatidial bristle (with Rab11^EP3017)

(Alone et al., 2005)

ommatidium

(Tiwari and Roy, 2009, Alone et al., 2005)

ommatidium (with Rab11^EP3017)

(Alone et al., 2005)

primary pigment cell

(Tiwari and Roy, 2009)

rhabdomere

(Tiwari and Roy, 2009)

Detailed Description

Statement

Reference

Mutants have defects in the pupal retina; cone and primary pigment cells are disorganised, and accumulation of actin is seen. The ommatidial lattice is disorganised. The arrangement of photoreceptor cell nuclei is irregular and most ommatidia contain less photoreceptor cells than normal. Interommatidial bristle cells are sometimes missing, or sometimes more than one interommatidial bristle cell is present in the same location. The total number of bristles in the eye is reduced compared to wild type.

The rhabdomeres of mutant pupal eyes do not have the normal trapezoidal shape and are fused/juxtaposed with other rhabdomeres. In mutant adult eyes, the rhabdomeres appear abnormally shaped or degenerated.

Homozygous adults have an abnormal pseudopupil under the light microscope.

Homozygous 45 hour pupal eyes have an increased number of dead cells compared to wild type.

(Tiwari and Roy, 2009)

Homozygotes show low viability and some survive only till larval, pupal or pharate adult stages, but viable adults are produced. The homozygous pharate adults and adult survivors have patches of malformed ommatidia, with fused ommatidia and missing bristles. In the most severely affected eyes, photoreceptor cells are hardly seen. Mutants also have antennal bristle abnormalities.

Third larval instar homozygous eye discs have an abnormal ommatidial arrangement and the axon bundles between the brain and eye discs are disorganised. There is an increase in the number of dying cells posterior to the morphogenetic furrow compared to wild type.

Adult Rab11^mo/Rab11^EP3017 escapers have eye defects including fused ommatidia and missing bristles.

Rab11^mo/Rab11^j2D1 flies have normal eyes.

(Alone et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Rab11^mo has increased cell death phenotype, suppressible by Scer\GAL4^GMR.PF/BacA\p35^UAS.cHa

(Tiwari and Roy, 2009)

Rab11^mo has visible phenotype, suppressible | partially by Scer\GAL4^GMR.PF/BacA\p35^UAS.cHa

(Tiwari and Roy, 2009)

Rab11^mo has visible phenotype, suppressible | partially by Df(3L)H99

(Tiwari and Roy, 2009)

Rab11^mo has increased cell death phenotype, suppressible by Df(3L)H99

(Tiwari and Roy, 2009)

Rab11^mo has visible phenotype, suppressible | partially by bsk¹/bsk[+]

(Tiwari and Roy, 2009)

Rab11^mo has visible phenotype, suppressible | partially by bsk²/bsk[+]

(Tiwari and Roy, 2009)

Rab11^mo has visible phenotype, suppressible | partially by msn¹⁷²/msn[+]

(Tiwari and Roy, 2009)

Enhancer of

Statement

Reference

Rab11^mo is an enhancer of visible phenotype of hid^GMR.PG

(Tiwari and Roy, 2009)

Rab11^mo is an enhancer of visible phenotype of grim^GMR.PH

(Tiwari and Roy, 2009)

Other

Statement

Reference

Rab11^mo, Scer\GAL4^GMR.PF, rpr^UAS.C has visible phenotype

(Tiwari and Roy, 2009)

Phenotype Manifest In

Suppressed by

Statement

Reference

Rab11^mo has eye phenotype, suppressible | partially by bsk¹/bsk[+]

(Tiwari and Roy, 2009)

Rab11^mo has eye phenotype, suppressible | partially by bsk²/bsk[+]

(Tiwari and Roy, 2009)

Rab11^mo has eye phenotype, suppressible | partially by msn¹⁷²/msn[+]

(Tiwari and Roy, 2009)

Rab11^mo has eye disc phenotype, suppressible by Scer\GAL4^GMR.PF/BacA\p35^UAS.cHa

(Tiwari and Roy, 2009)

Rab11^mo has eye phenotype, suppressible | partially by Scer\GAL4^GMR.PF/BacA\p35^UAS.cHa

(Tiwari and Roy, 2009)

Rab11^mo has eye phenotype, suppressible | partially by Df(3L)H99

(Tiwari and Roy, 2009)

Rab11^mo has eye disc phenotype, suppressible by Df(3L)H99

(Tiwari and Roy, 2009)

Enhancer of

Statement

Reference

Rab11^mo is an enhancer of eye phenotype of hid^GMR.PG

(Tiwari and Roy, 2009)

Rab11^mo is an enhancer of eye phenotype of grim^GMR.PH

(Tiwari and Roy, 2009)

Other

Statement

Reference

Rab11^mo, Scer\GAL4^GMR.PF, rpr^UAS.C has eye phenotype

(Tiwari and Roy, 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The eye defects seen in Rab11^mo homozygotes are partially suppressed by bsk¹/+, bsk²/+ or msn¹⁷²/+.

(Tiwari and Roy, 2009)

Xenogenetic Interactions

Statement

Reference

The increased cell death posterior to the morphogenetic furrow that is seen in Rab11^mo homozygous larval eye discs is significantly rescued if the animals are also expressing BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^GMR.PF.

The mutant eye phenotype of Rab11^mo homozygous adults is partially suppressed by expression of BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^GMR.PF.

The increased cell death posterior to the morphogenetic furrow that is seen in Rab11^mo homozygous larval eye discs is significantly rescued if the animals are also carrying Df(3L)H99.

The mutant eye phenotype of Rab11^mo homozygous adults is partially suppressed by Df(3L)H99.

(Tiwari and Roy, 2009)

Complementation and Rescue Data

Partially rescued by

Rab11^mo is partially rescued by Rab11^+t6.3.A

(Tiwari and Roy, 2009)

Rab11^mo is partially rescued by Rab11^+t6.3.A

(Alone et al., 2005)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Precise excision of the insertion reverts the mutant phenotype.

(Alone et al., 2005)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Rab11^mo

(Tiwari and Roy, 2009, Alone et al., 2005)

Name Synonyms

malformed ommatidia

(Alone et al., 2005)

Secondary FlyBase IDs

References (2)

Report Sections

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