FB2024_03 , released June 25, 2024
Allele: Dmel\MED27KK102849
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General Information
Symbol
Dmel\MED27KK102849
Species
D. melanogaster
Name
FlyBase ID
FBal0232050
Feature type
allele
Associated gene
Dmel\MED27
Associated Insertion(s)
Carried in Construct
P{KK102849}
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Keleman et al., 2009.8.5)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Keleman et al., 2009.8.5)
Mutagen
in vitro construct
(Keleman et al., 2009.8.5)
Progenitor genotype
Carried in construct
P{KK102849}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-KK102849
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of MED27KK102849 under the control of Scer\GAL4bam.T:Hsim\VP16 (in combination with a Dicer-2 transgene to enhance RNAi efficiency) results in partial meiotic arrest in spermatocytes.

      (Lu and Fuller, 2015)

      Expression via Scer\GAL4wor.PA and Scer\GAL80ase.PN prolongs the lifetime and prevents shrinkage of pupal neuroblasts (NBs) without having any obvious effects on larval NBs. These longer lived NBs continue dividing for a longer time and generate significantly more neuronal progeny than wild type.

      Cultured pupal neuroblasts (NBs) from flies expressing MED27KK102849 under the control of Scer\GAL4wor.PA and Scer\GAL80ase.PN do not reduce their net size (as wild type pupal NBs do) and are indistinguishable from larval NBs.

      Expression via Scer\GAL4ey-OK107 prevents proper differentiation of alpha/beta neurons in mushroom bodies, resulting in a smaller volume.

      Expression via Scer\GAL4insc-Mz1407 reduces the difference (increase) in oxygen consumption rates seen in wild type larval vs pupal brains. NADH levels are decreased, but overall energy/ATP levels are unchanged. Lactate levels are reduced in larvae and increased in pupae, effectively reducing the difference between the two stages, whereas lactate levels strongly decrease from larval to pupal stages in wild type.

      (Homem et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      MED27KK102849
      Name Synonyms
      Secondary FlyBase IDs
        References (3)