FlyBase Allele Report: Dmel\y[KK104196]

General Information

Symbol

Dmel\y^KK104196

Species

D. melanogaster

Name

FlyBase ID

FBal0232809

Feature type

allele

Associated gene

Dmel\y

Associated Insertion(s)

Carried in Construct

P{KK104196}

Also Known As

UAS-yellow-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK104196}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK104196

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body color | adult stage | male, with Scer\GAL4^dsx.KI

(Massey et al., 2019)

abnormal body color | adult stage | male, with Scer\GAL4^GMR42D04

(Massey et al., 2019)

abnormal body color | adult stage | male, with Scer\GAL4^GMR42D04, Scer\GAL80^nSyb.PS

(Massey et al., 2019)

abnormal mating | male, with Scer\GAL4^dsx.KI

(Massey et al., 2019)

abnormal mating | male, with Scer\GAL4^dsx-GAL4

(Massey et al., 2019)

abnormal mating | male, with Scer\GAL4^GMR42D04

(Massey et al., 2019)

abnormal mating | male, with Scer\GAL4^GMR42D04, Scer\GAL80^nSyb.PS

(Massey et al., 2019)

visible | adult stage, with Scer\GAL4^dsx.KI

(Massey et al., 2019)

visible | adult stage, with Scer\GAL4^GMR42D04

(Massey et al., 2019)

visible | adult stage, with Scer\GAL4^GMR42D04, Scer\GAL80^nSyb.PS

(Massey et al., 2019)

Phenotype Manifest In

abdominal tergite 5, with Scer\GAL4^dsx.KI

(Massey et al., 2019)

abdominal tergite 6, with Scer\GAL4^dsx.KI

(Massey et al., 2019)

sex comb, with Scer\GAL4^dsx.KI

(Massey et al., 2019)

sex comb, with Scer\GAL4^GMR42D04

(Massey et al., 2019)

sex comb, with Scer\GAL4^GMR42D04, Scer\GAL80^nSyb.PS

(Massey et al., 2019)

Detailed Description

Statement

Reference

The expression of y^KK104196 under the control of Scer\GAL4^GMR42D04 severely reduces male mating success, as males perform all precopulatory courtship behaviors normally but fail to copulate even after hours of attempts, and strongly reduced black melanin in the sex combs but not the abdomen. likewise, expression under the combined control of Scer\GAL4^GMR42D04 and Scer\GAL80^nSyb.PS reduces both male mating success, with males failing repeatedly to grasp the female abdomen with their sex combs when attempting to mount and copulate, and sex comb melanization.

The expression of y^KK104196 under the control of Scer\GAL4^dsx-GAL4, Scer\GAL4^dsx.KI, also severely reduces male mating success, as compared to controls. This phenotype is not observed when expression is under single control of either Scer\GAL4^fru.P1.D, Scer\GAL4^GMR57C10, Scer\GAL4^GMR27E08, Scer\GAL4^repo, Scer\GAL4^GMR40A05, Scer\GAL4^GMR41D01, Scer\GAL4^GMR42D02, Scer\GAL4^GMR41F06, Scer\GAL4^GMR41A01, Scer\GAL4^GMR39E06, Scer\GAL4^GMR42C06, Scer\GAL4^GMR40F04, Scer\GAL4^MJ49, Scer\GAL4^GMR33G09, Scer\GAL4^4G, Scer\GAL4^GMR29A02, Scer\GAL4^c315a, Scer\GAL4^{l(3)31-1-31-1}, Scer\GAL4^ple.PF, Scer\GAL4^Ddc.PL or Scer\GAL4^Hn.PU, or under the combined control of dsx^GAL4-DBD plus Scer\GAL4^AD.elav or dsx^GAL4-DBD plus Hsim\VP16^{AD-VGlut-OK371}. Using the Scer\GAL4^GMR40F03 driver does not yield reproducible effects.

The expression of y^KK104196 under the control of Scer\GAL4^dsx.KI partially reduces black melanin levels in the male A5 and A6 abdominal tergites.

The expression of y^KK104196 under the control of Scer\GAL4^y.PG results in a 'yellow' body color but does not affect male mating success.

(Massey et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v106068

P{KK104196}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

y^KK104196

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

Open Close