FlyBase Allele Report: Dmel\5-HT1A[KK108407]

General Information

Symbol

Dmel\5-HT1A^KK108407

Species

D. melanogaster

Name

FlyBase ID

FBal0235551

Feature type

allele

Associated gene

Dmel\5-HT1A

Associated Insertion(s)

Carried in Construct

P{KK108407}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK108407}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK108407

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cold stress response, with Scer\GAL4^Ilp2.215-3

(Luo et al., 2012)

abnormal cold stress response, with Scer\GAL4^Ilp2.PW

(Luo et al., 2012)

abnormal heat stress response, with Scer\GAL4^Ilp2.215-3

(Luo et al., 2012)

abnormal heat stress response, with Scer\GAL4^Ilp2.PW

(Luo et al., 2012)

abnormal locomotor behavior | adult stage, with Scer\GAL4^nSyb.PS

(Mohammad et al., 2016)

abnormal medium-term memory | adult stage, with Scer\GAL4^c739

(Muria et al., 2021)

abnormal memory, with Scer\GAL4^c739

(Lee et al., 2011)

abnormal memory, with Scer\GAL4^c739, Scer\GAL80^ts.αTub84B

(Lee et al., 2011)

abnormal memory, with Scer\GAL4^ey-OK107, Scer\GAL80^ts.αTub84B

(Lee et al., 2011)

abnormal starvation stress response, with Scer\GAL4^ey-OK107

(Luo et al., 2012)

abnormal starvation stress response, with Scer\GAL4^Ilp2.215-3

(Luo et al., 2012)

abnormal starvation stress response, with Scer\GAL4^Ilp2.PW

(Luo et al., 2012)

short lived, with Scer\GAL4^ey-OK107

(Luo et al., 2012)

short lived, with Scer\GAL4^Ilp2.215-3

(Luo et al., 2012)

short lived, with Scer\GAL4^Ilp2.PW

(Luo et al., 2012)

Phenotype Manifest In

Detailed Description

Statement

Reference

Temporal expression of 5-HT1A^KK108407 under the control of Scer\GAL4^c739 during adulthood (by using Gal80[ts]) decreases olfactory medium term memory (1h) when the experiment is carried out in crowded conditions (groups of 32 flies - socially facilitated memory).

(Muria et al., 2021)

Pan-neuronal expression of 5-HT1A^KK108407 in adult flies (using 31[o]C heat-induced Scer\GAL4^nSyb.PS in combination with Scer\GAL80^ts.αTub84B) does not significantly affect wall following behavior (posited measure of anxiety) in an arena, but does significantly decrease walking speed compared to non-induced (18[o]C) controls.

(Mohammad et al., 2016)

Starved adult male flies expressing 5-HT1A^KK108407 under the control of Scer\GAL4^Ilp2.PW display a significantly reduced median and maximal lifespan compared to controls.

Expression of 5-HT1A^KK108407 under the control of Scer\GAL4^ey-OK107 in adult male flies results in reduced resistance to starvation and a reduction in lifespan compared to controls.

Flies expressing 5-HT1A^KK108407 under the control of Scer\GAL4^Ilp2.PW display a decreased tolerance to heat. Likewise, these flies require increased time to recover from cold coma induced by exposure to 0[o]C for 4 hours.

Expression of 5-HT1A^KK108407 under the control of Scer\GAL4^ey-OK107 in adult male flies results in a significant reduction of lipid levels compared to control flies at 12 and 24 hours of starvation.

Adult flies (4-6 days old) expressing 5-HT1A^KK108407 under the control of Scer\GAL4^Ilp2.PW do not exhibit a significant difference in weight compared to controls.

(Luo et al., 2012)

Temporal down-regulation of 5-HT1A through expression of 5-HT1A^KK108407 in the whole of the mushroom body under the control of Scer\GAL4^ey-OK107 (and Scer\GAL80^ts.αTub84B) impairs 3 hour memory.

Knockdown of 5-HT1A expression in the α/β neurons in the mushroom body under the control of Scer\GAL4^c739 impairs the formation of 3 hour memory. Cold-induced anesthesia further suppresses the 3 hour memory caused by down-regulation of 5-HT1A in α/β neurons. The presence of Scer\GAL80^ts.αTub84B, to minimize any developmental contribution, does not affect the impairment of 3 hour memory.

Knockdown of 5-HT1A expression in the α'/β' neurons (Scer\GAL4^c305a) or the &ygr; neurons (Scer\GAL4¹³¹⁰⁹) in the mushroom body does not affect the formation of 3 hour memory.

(Lee et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v106094

P{KK108407}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

5-HT1A^KK108407

d5-HT1A^KK108407

(Mohammad et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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