FlyBase Allele Report: Dmel\POSH[74]

General Information

Symbol

Dmel\POSH⁷⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0240638

Feature type

allele

Associated gene

Dmel\POSH

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

hypomorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Zhang et al., 2010)

hypomorphic allele - molecular evidence

(Zhang et al., 2010)

Mutagen

P-element activity

(Tsuda et al., 2005)

Progenitor genotype

P{EP}POSH^EP1206

(Tsuda et al., 2005)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a deletion of POSH sequences that includes the start codon and extends 1282bp into the coding region.

(Tsuda et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:17,568,794..17,570,075 [+]

Comment:

Inferred boundaries of a 1,282 bp deletion resulting from the imprecise excision of P{EP}POSH^EP1206, which removes the ATG and first half of the coding sequence of POSH.

(Tsuda et al., 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates frontotemporal dementia

modeled by Hsap\CHMP2B^Intron5.UAS

(West et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response | adult stage

(Tsuda et al., 2005)

abnormal locomotor behavior | third instar larval stage

(West et al., 2018)

melanotic mass phenotype

(Tsuda et al., 2005)

Phenotype Manifest In

melanotic mass

(Tsuda et al., 2005)

Detailed Description

Statement

Reference

POSH⁷⁴ homozygous mutants do not show any synaptic overgrowth at larval neuromuscular junction (assessed by the number of boutons and NMJ length) but the crawling velocity of third instar larvae is significantly reduced compared to wild-type.

(West et al., 2018)

Most homozygotes survive embryogenesis.

Older cultures of POSH⁷⁴ stocks often contain dead larvae and pupae that are heavily melanised.

POSH⁷⁴ adults injected with E. coli show significantly reduced viability compared to control flies.

(Tsuda et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

POSH⁷⁴/POSH⁷⁴ is a suppressor of abnormal locomotor behavior | third instar larval stage phenotype of Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^nSyb.PS

(West et al., 2018)

POSH⁷⁴/POSH⁷⁴ is a suppressor of lethal - all die before end of P-stage phenotype of Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^nSyb.PS

(West et al., 2018)

Phenotype Manifest In

Suppressor of

Statement

Reference

POSH⁷⁴/POSH⁷⁴ is a suppressor of neuromuscular junction | third instar larval stage phenotype of Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^nSyb.PS

(West et al., 2018)

POSH⁷⁴/POSH⁷⁴ is a suppressor of NMJ bouton | third instar larval stage phenotype of Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^nSyb.PS

(West et al., 2018)

POSH⁷⁴ is a suppressor of eye phenotype of POSH^UAS.cSa, Scer\GAL4^GMR.PF, egr^UAS.cMa

(Tsuda et al., 2010)

POSH⁷⁴ is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, egr^UAS.cMa

(Tsuda et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

A POSH⁷⁴ background partially suppresses the small eye phenotype found upon expression of egr^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PF.

(Tsuda et al., 2010)

Xenogenetic Interactions

Statement

Reference

The neuromuscular junction synaptic overgrowth and larval locomotion deficits along with the abnormal brain accumulations of POSH protein induced by Scer\GAL4^nSyb.PS-driven expression of Hsap\CHMP2B^Intron5.UAS is ameliorated by POSH⁷⁴ homozygosity, which also decreases the elevated cell death in the larval brain.

(West et al., 2018)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

58808

w^*; P{FRT(w^hs)}G13 POSH⁷⁴

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

POSH⁷⁴

(West et al., 2018, West et al., 2015, Tsuda et al., 2010, Tsuda et al., 2005)

posh ⁷⁴

(Zhang et al., 2010)

posh⁷⁴

(Zhang et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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