FlyBase Allele Report: Dmel\NT1[41]

In the CNS of homozygous NT1⁴¹ null mutant embryos, apoptosis levels are comparable to wild-type at stages 13/15, and there are no axon guidance defects. At embryonic stage 17, NT1⁴¹, NT1⁴¹/NT1⁵⁵ and NT1⁴¹/Df(3L)ED4342 null mutant embryos show a significant increase in apoptosis in the CNS.

NT1⁴¹, NT1⁴¹/NT1⁵⁵ and NT1⁴¹/Df(3L)ED4342 mutant embryos exhibit a significant increase in the incidence of misrouting phenotypes in ISNb/d and SN fascicles compared to wild-type, including effects in more than one projection per hemisegment (e.g. misrouting plus loss).

Loss of NT1 in NT1⁴¹ mutants affect ISNb/d projections more severely than SNa projections.

Targeted expression of NT1^{dsRNA.pro.Scer\UAS} in the CNS midline (under the control of Scer\GAL4^sim.PS) in a NT1⁴¹ heterozygous background results in a significant increase in apoptosis throughout the CNS cortex compared to controls at embryonic stage 17.

Targeted expression of NT1^{dsRNA.CK.Scer\UAS} in the CNS midline (under the control of Scer\GAL4^sim.PS) in a NT1⁴¹ heterozygous background results in a significant increase in apoptosis throughout the CNS cortex compared to controls at embryonic stage 17.

Targeted expression of NT1^{dsRNA.CK.Scer\UAS} in the muscle (under the control of Scer\GAL4^how-24B) in a NT1⁴¹ heterozygous background results in a significant increase in the incidence of misrouting phenotypes in ISNb/d and SN fascicles compared to wild-type, including effects in more than one projection per hemisegment (e.g. misrouting plus loss).

In the CNS of homozygous NT1^41.Δ.w null mutant embryos, apoptosis levels are comparable to wild-type at stages 13/15, and there are no axon guidance defects. At embryonic stage 17, NT1^41.Δ.w, NT1^41.Δ.w/NT1^55.Δ.w and NT1^41.Δ.w/Df(3L)ED4342 null mutant embryos show a significant increase in apoptosis in the CNS.

NT1^41.Δ.w, NT1^41.Δ.w/NT1^55.Δ.w and NT1^41.Δ.w/Df(3L)ED4342 mutant embryos exhibit a significant increase in the incidence of misrouting phenotypes in ISNb/d and SN fascicles compared to wild-type, including effects in more than one projection per hemisegment (e.g. misrouting plus loss).

Loss of NT1 in NT1^41.Δ.w mutants affect ISNb/d projections more severely than SNa projections.

Targeted expression of NT1^{IR.pro.Scer\UAS} in the CNS midline (under the control of Scer\GAL4^sim.PS) in a NT1^41.Δ.w heterozygous background results in a significant increase in apoptosis throughout the CNS cortex compared to controls at embryonic stage 17.

Targeted expression of NT1^{IR.CK.Scer\UAS} in the CNS midline (under the control of Scer\GAL4^sim.PS) in a NT1^41.Δ.w heterozygous background results in a significant increase in apoptosis throughout the CNS cortex compared to controls at embryonic stage 17.

Targeted expression of NT1^{IR.CK.Scer\UAS} in the muscle (under the control of Scer\GAL4^how-24B) in a NT1^41.Δ.w heterozygous background results in a significant increase in the incidence of misrouting phenotypes in ISNb/d and SN fascicles compared to wild-type, including effects in more than one projection per hemisegment (e.g. misrouting plus loss).

(Zhu et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

NT1⁴¹, spz² has abnormal neuroanatomy phenotype, enhanceable by spz5^e03444

(Zhu et al., 2008)

NT1⁴¹ has abnormal neuroanatomy phenotype, enhanceable by spz²/spz5^e03444

(Zhu et al., 2008)

NT1⁴¹, spz5^e03444 has abnormal neuroanatomy phenotype, enhanceable by spz²

(Zhu et al., 2008)

Df(3L)ED4342/NT1⁴¹ has abnormal neuroanatomy phenotype, enhanceable by spz5^e03444

(Zhu et al., 2008)

NOT Enhanced by

Statement

Reference

NT1⁴¹ has abnormal neuroanatomy phenotype, non-enhanceable by spz²

(Zhu et al., 2008)

NT1⁴¹ has increased cell death phenotype, non-enhanceable by spz²

(Zhu et al., 2008)

Suppressed by

Statement

Reference

NT1⁴¹, spz5^e03444 has lethal phenotype, suppressible by Scer\GAL4^elav.PU/kek2^{UAS.mRFP(Unk)}

(Ulian-Benitez et al., 2017)

NT1⁴¹, spz5^e03444 has lethal phenotype, suppressible by Scer\GAL4^elav.PU/kek3^{UAS.mRFP(Unk)}

(Ulian-Benitez et al., 2017)

NT1⁴¹, spz5^e03444 has lethal phenotype, suppressible by kek5^{UAS.mRFP(Unk)}/Scer\GAL4^elav.PU

(Ulian-Benitez et al., 2017)

NT1⁴¹, spz5^e03444 has lethal phenotype, suppressible by Scer\GAL4^elav.PU/kek6^{UAS.mRFP(Unk)}

(Ulian-Benitez et al., 2017)

NT1⁴¹, spz5^e03444 has some die during embryonic stage | heat sensitive phenotype, suppressible by Toll-7^{C993Y.UAS.Tag:FLAG}/Scer\GAL4^elav-C155

(McIlroy et al., 2013)

NT1⁴¹, spz5^e03444 has some die during embryonic stage | heat sensitive phenotype, suppressible by Scer\GAL4^elav-C155/Toll-7^{ΔLRR.UAS.Tag:HA}

(McIlroy et al., 2013)

NT1⁴¹, spz5^e03444 has some die during embryonic stage | heat sensitive phenotype, suppressible by Scer\GAL4^elav-C155/Tl^10b.UAS.cYa

(McIlroy et al., 2013)

NT1⁴¹, Toll-6²⁶ has some die during embryonic stage | heat sensitive phenotype, suppressible by Toll-7^{C993Y.UAS.Tag:FLAG}/Scer\GAL4^Toll-7

(McIlroy et al., 2013)

NT1⁴¹, Toll-6²⁶ has some die during embryonic stage | heat sensitive phenotype, suppressible by Scer\GAL4^Toll-7/Toll-6^{C1020Y.UAS.Tag:FLAG}

(McIlroy et al., 2013)

NT1⁴¹, spz5^e03444 has some die during embryonic stage | heat sensitive phenotype, suppressible by Scer\GAL4^elav-C155/Toll-6^{ΔLRR.UAS.Tag:HA}

(McIlroy et al., 2013)

NT1⁴¹ has increased cell death phenotype, suppressible by Scer\GAL4^elav-C155/spz^act.UAS

(Zhu et al., 2008)

NOT suppressed by

Statement

Reference

NT1⁴¹, spz5^e03444 has lethal phenotype, non-suppressible by Scer\GAL4^elav.PU/kek1^{UAS.mRFP(Unk)}

(Ulian-Benitez et al., 2017)

NT1⁴¹, spz5^e03444 has lethal phenotype, non-suppressible by Scer\GAL4^elav.PU/kek4^{UAS.mRFP(Unk)}

(Ulian-Benitez et al., 2017)

Enhancer of

Statement

Reference

NT1⁴¹ is an enhancer of abnormal neuroanatomy phenotype of spz5^e03444, spz²

(Zhu et al., 2008)

NT1⁴¹/spz5^e03444 is an enhancer of abnormal neuroanatomy phenotype of spz²

(Zhu et al., 2008)

spz²/NT1⁴¹ is an enhancer of abnormal neuroanatomy phenotype of spz5^e03444

(Zhu et al., 2008)

Df(3L)ED4342/NT1⁴¹ is an enhancer of abnormal neuroanatomy phenotype of spz5^e03444

(Zhu et al., 2008)

NOT Enhancer of

Statement

Reference

NT1⁴¹ is a non-enhancer of increased cell death phenotype of spz²

(Zhu et al., 2008)

Other

Statement

Reference

NT1⁴¹, spz5^e03444 has lethal phenotype

(Ulian-Benitez et al., 2017)

NT1⁴¹, Toll-7^P114 has some die during embryonic stage | heat sensitive phenotype

(McIlroy et al., 2013)

NT1⁴¹, spz5^e03444 has some die during embryonic stage | heat sensitive phenotype

(McIlroy et al., 2013)

Df(3L)Exel6092, NT1⁴¹ has some die during embryonic stage | heat sensitive phenotype

(McIlroy et al., 2013)

NT1⁴¹, Toll-6²⁶ has some die during embryonic stage | heat sensitive phenotype

(McIlroy et al., 2013)

NT1⁴¹, spz² has lethal phenotype

(Zhu et al., 2008)

NT1⁴¹, spz5^e03444, spz² has abnormal locomotor rhythm phenotype

(Zhu et al., 2008)

Df(3L)ED4342/NT1⁴¹, spz5^e03444, spz² has abnormal locomotor rhythm phenotype

(Zhu et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

NT1⁴¹, spz² has larval segmental nerve phenotype, enhanceable by spz5^e03444

(Zhu et al., 2008)

NT1⁴¹, spz² has larval transverse nerve phenotype, enhanceable by spz5^e03444

(Zhu et al., 2008)

NT1⁴¹ has larval intersegmental nerve branch ISNb of A1-7 phenotype, enhanceable by spz²/spz5^e03444

(Zhu et al., 2008)

NT1⁴¹ has larval intersegmental nerve branch ISNd of A1-7 phenotype, enhanceable by spz²/spz5^e03444

(Zhu et al., 2008)

NT1⁴¹ has larval segmental nerve branch SNa of A1-7 phenotype, enhanceable by spz²/spz5^e03444

(Zhu et al., 2008)

NT1⁴¹ has larval segmental nerve phenotype, enhanceable by spz²/spz5^e03444

(Zhu et al., 2008)

NT1⁴¹ has larval transverse nerve phenotype, enhanceable by spz²/spz5^e03444

(Zhu et al., 2008)

NT1⁴¹, spz5^e03444 has larval intersegmental nerve branch ISNb of A1-7 phenotype, enhanceable by spz²

(Zhu et al., 2008)

NT1⁴¹, spz5^e03444 has larval intersegmental nerve branch ISNd of A1-7 phenotype, enhanceable by spz²

(Zhu et al., 2008)

NT1⁴¹, spz5^e03444 has larval segmental nerve phenotype, enhanceable by spz²

(Zhu et al., 2008)

NT1⁴¹, spz5^e03444 has larval transverse nerve phenotype, enhanceable by spz²

(Zhu et al., 2008)

Df(3L)ED4342/NT1⁴¹ has larval intersegmental nerve branch ISNb of A1-7 phenotype, enhanceable by spz5^e03444

(Zhu et al., 2008)

Df(3L)ED4342/NT1⁴¹ has larval intersegmental nerve branch ISNd of A1-7 phenotype, enhanceable by spz5^e03444

(Zhu et al., 2008)

Df(3L)ED4342/NT1⁴¹ has larval segmental nerve phenotype, enhanceable by spz5^e03444

(Zhu et al., 2008)

NT1⁴¹, spz² has larval intersegmental nerve branch ISNb of A1-7 phenotype, enhanceable by spz5^e03444

(Zhu et al., 2008)

NT1⁴¹, spz² has larval intersegmental nerve branch ISNd of A1-7 phenotype, enhanceable by spz5^e03444

(Zhu et al., 2008)

NOT Enhanced by

Statement

Reference

NT1⁴¹ has larval segmental nerve phenotype, non-enhanceable by spz²

(Zhu et al., 2008)

Enhancer of

Statement

Reference

NT1⁴¹ is an enhancer of larval intersegmental nerve branch ISNb of A1-7 phenotype of spz5^e03444, spz²

(Zhu et al., 2008)

NT1⁴¹ is an enhancer of larval intersegmental nerve branch ISNd of A1-7 phenotype of spz5^e03444, spz²

(Zhu et al., 2008)

NT1⁴¹ is an enhancer of larval segmental nerve phenotype of spz5^e03444, spz²

(Zhu et al., 2008)

NT1⁴¹ is an enhancer of larval transverse nerve phenotype of spz5^e03444, spz²

(Zhu et al., 2008)

NT1⁴¹/spz5^e03444 is an enhancer of larval intersegmental nerve branch ISNb of A1-7 phenotype of spz²

(Zhu et al., 2008)

NT1⁴¹/spz5^e03444 is an enhancer of larval intersegmental nerve branch ISNd of A1-7 phenotype of spz²

(Zhu et al., 2008)

NT1⁴¹/spz5^e03444 is an enhancer of larval segmental nerve branch SNa of A1-7 phenotype of spz²

(Zhu et al., 2008)

NT1⁴¹/spz5^e03444 is an enhancer of larval segmental nerve phenotype of spz²

(Zhu et al., 2008)

NT1⁴¹/spz5^e03444 is an enhancer of larval transverse nerve phenotype of spz²

(Zhu et al., 2008)

spz²/NT1⁴¹ is an enhancer of larval intersegmental nerve branch ISNb of A1-7 phenotype of spz5^e03444

(Zhu et al., 2008)

spz²/NT1⁴¹ is an enhancer of larval intersegmental nerve branch ISNd of A1-7 phenotype of spz5^e03444

(Zhu et al., 2008)

spz²/NT1⁴¹ is an enhancer of larval segmental nerve branch SNa of A1-7 phenotype of spz5^e03444

(Zhu et al., 2008)

spz²/NT1⁴¹ is an enhancer of larval segmental nerve phenotype of spz5^e03444

(Zhu et al., 2008)

spz²/NT1⁴¹ is an enhancer of larval transverse nerve phenotype of spz5^e03444

(Zhu et al., 2008)

Df(3L)ED4342/NT1⁴¹ is an enhancer of larval intersegmental nerve branch ISNb of A1-7 phenotype of spz5^e03444

(Zhu et al., 2008)

Df(3L)ED4342/NT1⁴¹ is an enhancer of larval intersegmental nerve branch ISNd of A1-7 phenotype of spz5^e03444

(Zhu et al., 2008)

Df(3L)ED4342/NT1⁴¹ is an enhancer of larval segmental nerve phenotype of spz5^e03444

(Zhu et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

NT1⁴¹, spz5^e03444 double homozygosity leads to near lethality, which is rescued by Scer\GAL4^elav.PU-driven expression of kek3^{UAS.mRFP(Unk)}, kek5^{UAS.mRFP(Unk)} (leading to a small but significant increase in viability), kek2^{UAS.mRFP(Unk)} or kek6^{UAS.mRFP(Unk)} (leading to near full viability), but not kek1^{UAS.mRFP(Unk)} or kek4^{UAS.mRFP(Unk)}.

(Ulian-Benitez et al., 2017)

DA1 and VA1d projection neuron dendrites and VA1d olfactory receptor neuron axons target normally in NT1⁴¹ Df(3L)spz5^AW18 double mutant flies.

(Ward et al., 2015)

The SNa axonal phenotype of spz² NT1⁴¹ double-mutant embryos is epistatic to spz.

Loss of both NT1 and spz5 in spz5^e03444 NT1⁴¹ double-mutant embryos results in an increase in apoptosis compared to each of the single mutants, revealing redundant or complementary functions in the control of neuronal survival.

Levels of apoptosis do not increase further in spz² NT1⁴¹ double mutants (compared to spz² mutants).

Whereas homozygous spz² flies can eclose as adults, the NT1⁴¹ spz² double mutants are completely lethal (100% penetrance).

Expression of spz^act.Scer\UAS in NT1⁴¹ mutant embryos partially rescues apoptosis levels, but does not rescue the NT1⁴¹ mutant phenotype.

Viability is affected in NT1⁴¹ spz5^e03444 double-mutants: in homozygosis, double mutant flies are viable (although some larval lethality, as well as when in trans over NT1⁴¹ Df(3L)Exel6092, is observed), but NT1⁴¹ spz5^e03444/In(3LR)TM6B flies do not produce homozygous progeny at 18[o]C, suggesting unsuccessful larval competition.

The penetrance of ISNb/d targeting defects increases in spz5^e03444 NT1⁴¹ / Df(3L)Exel6092 NT1⁴¹ double-mutant embryos, although not significantly.

The penetrance of both SNa and ISNb/d targeting defects increases in spz5^e03444 NT1⁴¹ spz², compared to the double or single mutants. In the triple mutants, misrouting phenotypes can be very dramatic, and there are cases of loss of all ISNb/d motor axons (not seen in single mutants). Misrouting of the transverse nerve (TN) can be very dramatic in triple mutants, although milder effects in this nerve occur with comparable penetrance in all genotypes (~10%). Misroutings of ISN are negligible in single and double mutants, but they increase and can be dramatic in triple-mutant embryos (12.7%).

NT1⁴¹ spz5^e03444 / NT1⁴¹ Df(3L)Exel6092 double mutant flies display a range of behavioral phenotypes including: inability to estimate the location of a petri dish rim, falling off a petri dish rim, sluggishness, inability to climb, and wobbling.

The SNa axonal phenotype of spz² NT1^41.Δ.w double-mutant embryos is epistatic to spz.

Loss of both NT1 and spz5 in spz5^e03444 NT1^41.Δ.w double-mutant embryos results in an increase in apoptosis compared to each of the single mutants, revealing redundant or complementary functions in the control of neuronal survival.

Levels of apoptosis do not increase further in spz² NT1^41.Δ.w double mutants (compared to spz² mutants).

Whereas homozygous spz² flies can eclose as adults, the NT1^41.Δ.w spz² double mutants are completely lethal (100% penetrance).

Expression of spz^act.Scer\UAS in NT1^41.Δ.w mutant embryos partially rescues apoptosis levels, but does not rescue the NT1^41.Δ.w mutant phenotype.

Viability is affected in NT1^41.Δ.w spz5^e03444 double-mutants: in homozygosis, double mutant flies are viable (although some larval lethality, as well as when in trans over NT1^41.Δ.w Df(3L)Exel6092, is observed), but NT1^41.Δ.w spz5^e03444/In(3LR)TM6B flies do not produce homozygous progeny at 18[o]C, suggesting unsuccessful larval competition.

The penetrance of ISNb/d targeting defects increases in spz5^e03444 NT1^41.Δ.w / Df(3L)Exel6092 NT1^41.Δ.w double-mutant embryos, although not significantly.

The penetrance of both SNa and ISNb/d targeting defects increases in spz5^e03444 NT1^41.Δ.w spz², compared to the double or single mutants. In the triple mutants, misrouting phenotypes can be very dramatic, and there are cases of loss of all ISNb/d motor axons (not seen in single mutants). Misrouting of the transverse nerve (TN) can be very dramatic in triple mutants, although milder effects in this nerve occur with comparable penetrance in all genotypes (~10%). Misroutings of ISN are negligible in single and double mutants, but they increase and can be dramatic in triple-mutant embryos (12.7%).

NT1^41.Δ.w spz5^e03444 / NT1^41.Δ.w Df(3L)Exel6092 double mutant flies display a range of behavioral phenotypes including: inability to estimate the location of a petri dish rim, falling off a petri dish rim, sluggishness, inability to climb, and wobbling.

(Zhu et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

NT1⁴¹ is rescued by Scer\GAL4^elav-C155/NT1^Cysknot.UAS

(Zhu et al., 2008)

Comments

Expression of NT1^{Cysknot.Scer\UAS} in all neurons (under the control of Scer\GAL4^elav-C155) in embryos mutant for NT1^41.Δ.w leads to a significant reduction in apoptosis compared to NT1^41.Δ.w mutants.

Expression of NT1^{Cysknot.Scer\UAS} in all neurons (under the control of Scer\GAL4^elav-C155) in embryos mutant for NT1⁴¹ leads to a significant reduction in apoptosis compared to NT1⁴¹ mutants.

(Zhu et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

64069

y¹ w^*; Df(3L)spz5^AW18 P+PBac{XP3.WH3}spz5-AW18 TI{TI}NT1⁴¹/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

DNT1⁴¹

(Ulian-Benitez et al., 2017, McIlroy et al., 2013, Akhouayri et al., 2011, Zhu et al., 2008)

NT1^41.Δ.w

NT1⁴¹

dNT1⁴¹

(Ward et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (6)

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