FB2024_03 , released June 25, 2024
Allele: Dmel\APC7RNAi.WIZ.UAS
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General Information
Symbol
Dmel\APC7RNAi.WIZ.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0241856
Feature type
allele
Associated gene
Dmel\APC7
Associated Insertion(s)
Carried in Construct
P{UAS-APC7.RNAi}
Key Links
Transgenic product class
RNAi_reagent
(Pal et al., 2007)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Pal et al., 2007)
Mutagen
in vitro construct
(Pal et al., 2007)
Progenitor genotype
Carried in construct
P{UAS-APC7.RNAi}
Cytology
Description

UAS regulatory sequences drive expression of an inverted repeat of a 917bp fragment of APC7 exon 3 (the two copies of the inverted repeat are separated by a w intron spacer).

(Pal et al., 2007)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
APC7
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Brain neuroblasts from third instar larvae expressing Apc7dsRNA.WIZ.Scer\UAS under the control of Scer\GAL4Act5C lack any sign of prometaphase or metaphase-like arrest. the chromosomes appear somewhat more tangled than normal and show signs of uneven condensation, together with frequent chromosome bridges in anaphase. The number of cells in metaphase remains low, but the proportion of anaphase and telophase figures approximates the number of cells in prometaphase and metaphase, resulting in a mitotic index that is similar to wild type and a metaphase:anaphase ratio that is lower than wild type.

      (Pal et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Animals co-expressing Apc7dsRNA.WIZ.Scer\UAS and cdc23dsRNA.WIZ.Scer\UAS under the control of Scer\GAL4Act5C show greatly increased larval lethality and occurrence of melanotic tumours compared to animals expressing cdc23dsRNA.WIZ.Scer\UAS singly under the control of Scer\GAL4Act5C. There is also a shift in pupal lethality towards earlier stages in the double mutant animals.

      The mitotic index of brain neuroblasts in larvae co-expressing Apc7dsRNA.WIZ.Scer\UAS and cdc23dsRNA.WIZ.Scer\UAS under the control of Scer\GAL4Act5C is significantly higher than that in larvae expressing cdc23dsRNA.WIZ.Scer\UAS singly under the control of Scer\GAL4Act5C.

      (Pal et al., 2007)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      APC7RNAi.WIZ.UAS
      APC7dsRNA.WIZ.Scer\UAS
      APC7dsRNA.WIZ.UAS
      Apc7dsRNA.WIZ.Scer\UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (2)