No abrogation of the endocycle is observed when cdc16dsRNA.WIZ.Scer\UAS is expressed in 'flip out' clones in salivary glands.
Sporadic occurrence of melanotic tissue is seen in third instar larvae expressing cdc16dsRNA.WIZ.Scer\UAS under the control of Scer\GAL4Act5C.
Animals expressing cdc16dsRNA.WIZ.Scer\UAS under the control of Scer\GAL4Act5C die during the first part of the phanerocephalic pupal (malpighian tubules migrating) stage of P5(i) (some lethality is also seen during the third instar larval stage).
Brain neuroblasts from third instar larvae expressing cdc16dsRNA.WIZ.Scer\UAS under the control of Scer\GAL4Act5C show metaphase-like arrest with overcondensed chromosomes. the chromosomes in most of the arrested cells appear scattered, while in about 10% of mitotic cells the chromosomes are locked at the metaphase plate. Some cells appear polyploid. The mitotic index is almost double that of wild type. The metaphase:anaphase ratio is also increased compared to wild type. The larval brains show higher levels of apoptosis than controls.
Animals co-expressing cdc16dsRNA.WIZ.Scer\UAS and cdc23dsRNA.WIZ.Scer\UAS under the control of Scer\GAL4Act5C show greatly increased larval lethality and occurrence of melanotic tumours compared to animals expressing either cdc16dsRNA.WIZ.Scer\UAS or cdc23dsRNA.WIZ.Scer\UAS singly under the control of Scer\GAL4Act5C. There is also a shift in pupal lethality towards earlier stages in the double mutant animals.
The mitotic index of brain neuroblasts in larvae co-expressing cdc16dsRNA.WIZ.Scer\UAS and cdc23dsRNA.WIZ.Scer\UAS under the control of Scer\GAL4Act5C is significantly higher than that in larvae expressing either cdc16dsRNA.WIZ.Scer\UAS or cdc23dsRNA.WIZ.Scer\UAS singly under the control of Scer\GAL4Act5C.
