FB2024_03 , released June 25, 2024
Allele: Dmel\sNPF-RDN.UAS
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General Information
Symbol
Dmel\sNPF-RDN.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0244780
Feature type
allele
Associated gene
Dmel\sNPF-R
Associated Insertion(s)
Carried in Construct
P{UAS-sNPF-R.DN}
Also Known As
UAS-sNPFR1-DN
Key Links
Transgenic product class
dominant_negative_variant
(Lee et al., 2008)
inframe_deletion
(Lee et al., 2008)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Lee et al., 2008)
inframe_deletion
(Lee et al., 2008)
Mutagen
in vitro construct
(Lee et al., 2008)
Progenitor genotype
Carried in construct
P{UAS-sNPF-R.DN}
Cytology
Description

UAS drives expression of a cDNA encoding the third intracellular domain of sNPF-R, which acts as a dominant-negative mutant.

(Lee et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
sNPF-R
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of sNPF-RDN.UAS under the control of Scer\GAL41003.3 and Scer\GAL80tsh-GAL80 or Scer\GAL4ppk.PG, but not Scer\GAL4GMR72F11, in larvae significantly reduces nociceptive movement responses following stimulation with a von Frey filament, compared to controls; in the case of Scer\GAL41003.3 and Scer\GAL80tsh-GAL80, or Scer\GAL4ppk.PG, there is no effect on the response to gentle touch, compared to controls; expression under the control of Scer\GAL4ppk.PG does not affect the latency of rolling in response to high temperature (local) stimulation, compared to controls.

      (Hu et al., 2017)

      Flies expressing sNPF-RDN.Scer\UAS under the control of Scer\GAL4sNPF-R.2.5 show significantly decreased food consumption compared to controls.

      (Hong et al., 2012)

      Expression of sNPF-RScer\UAS.DN using Scer\GAL4MJ94 has little effect on size.

      Expression of sNPF-RScer\UAS.DN using Scer\GAL4Ilp2.PR decreases body size by 10%.

      (Lee et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      sNPF-RDN.Scer\UAS
      sNPF-RDN.UAS
      sNPF-RScer\UAS.DN
      Name Synonyms
      Secondary FlyBase IDs
        References (4)