ScerGAL4amn-NP2397 drives expression in mushroom body extrinsic neurons, including the mushroom body anterior inferior medial cell and dopaminergic PAM neurons, including dopaminergic PAM neurons 13 and 14, that innervate mushroom body gamma lobe slice 3, mushroom body gamma lobe slice 4, mushroom body gamma lobe slice 5, mushroom body beta' lobe slice 1 and mushroom body beta' lobe slice 2. Expression partially overlaps with that driven by EcollexAGMR24E12 in 12 dopaminergic PAM neurons 13 and 14.
Projections of the mushroom body anterior inferior medial neurons innervate the anterior stratum of the beta' lobe segment 1, and/or throughout the middle and posterior strata of segment 2.
Inhibition (using Hsap\KCNJ2UAS.EGFP) of Scer\GAL4NP2397 neurons, in combination with a Gal80[ts] transgene to restrict expression to 1 day prior to training and testing, significantly improves long term (1 hour, 3 hour and 6 hour), but not immediate short term, aversive olfactory memory, compared to controls, but this is no longer significant 24 hours after training; 3 hour memory following a 2 min cold shock is similar to that of controls; the 3 hour memory improvement still occurs when inhibition is restricted to a smaller subset of Scer\GAL4NP2397 cells using Scer\GAL80ple.PK, Scer\GAL80VGlut.PV or Scer\GAL80R48B04, but not Scer\GAL80R58E02 or Scer\GAL80R24E12; there is no effect on reversal learning, shock reactivity or olfactory acuity, compared to controls.
Inhibition (using Hsap\KCNJ2UAS.EGFP) of the combined set of Scer\GAL4GMR76B09 neurons and Scer\GAL4NP2397 neurons, in combination with a Gal80[ts] transgene to restrict expression to 1 day prior to training and testing, significantly improves long term aversive olfactory memory across multiple time points, to a greater extent than either group alone (additive), compared to controls; there is no effect on reversal learning, compared to controls.
Inhibition (using Ctet\TeTxLCTNT.UAS) of Scer\GAL4NP2397 neurons, in combination with a Gal80[ts] transgene to restrict expression to 1 day prior to training and testing, significantly improves 3 hour aversive olfactory memory, compared to controls.
Inhibition (using shi1.UAS at 30[o]C) of Scer\GAL4NP2397 neurons from 30 min prior to training onwards does not affect 3 hour aversive olfactory memory, compared to controls.
Thermogenetic activation (using TrpA1UAS.cUa at 32[o]C) of Scer\GAL4NP2397 neurons for the 20 min immediately after training, but not the 20 min immediately before training, significantly reduces 3 hour aversive olfactory memory, compared to controls.
