FlyBase Allele Report: Dmel\TrpA1[UAS.cUa]

General Information

Symbol

Dmel\TrpA1^UAS.cUa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Unknown

FlyBase ID

FBal0291243

Feature type

allele

Associated gene

Dmel\TrpA1

Associated Insertion(s)

Carried in Construct

P{UAS-TrpA1.U}

Also Known As

UAS-dTrpA1, UAS-TrpA1, UAS-DmTRPA1

Key Links

Transgenic product class

wild_type

(Yuan et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Yuan et al., 2011)

Mutagen

in vitro construct

(Yuan et al., 2011)

Progenitor genotype

Carried in construct

P{UAS-TrpA1.U}

Cytology

Description

UAS regulatory sequences drive expression of TrpA1.

(Yuan et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

TrpA1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal chemosensory behavior | adult stage | heat sensitive, with Hsap\RELA^AD.R76F05, Scer\GAL4^DBD.R23C12

(Lewis et al., 2015)

abnormal chemosensory behavior | adult stage | heat sensitive, with Hsap\RELA^AD.R76F05, Scer\GAL4^DBD.R80G12

(Lewis et al., 2015)

abnormal copulation, with Scer\GAL4^{CCKLR-17D3-2A-GAL4}

(Wang et al., 2022)

abnormal copulation, with Scer\GAL4^Dsk-2A-GAL4

(Wang et al., 2022)

abnormal copulation, with Scer\GAL4^GMR71G01

(Wang et al., 2022)

abnormal copulation | somatic clone - tissue specific, with Scer\GAL4^{GluRIA-2A-GAL4}

(Wang et al., 2022)

abnormal feeding behavior | heat sensitive, with Scer\GAL4^NPF.1

(Chung et al., 2017)

abnormal feeding behavior | heat sensitive, with Scer\GAL4^NPF.1, Scer\GAL80^cry.PS

(Chung et al., 2017)

abnormal feeding behavior | larval stage, with Scer\GAL4^ple.PF

(Bjordal et al., 2014)

abnormal mating behavior | female limited, with Scer\GAL4^Tdc2.PC

(Rezával et al., 2014)

abnormal mating behavior | female limited | heat sensitive, with Scer\GAL4^dsx.KI

(Kimura et al., 2015)

abnormal memory | adult stage | heat sensitive, with Scer\GAL4^GMR76B09

(Shuai et al., 2015)

abnormal memory | adult stage | heat sensitive, with Scer\GAL4^NP2397

(Shuai et al., 2015)

abnormal neuroanatomy | somatic clone, with Scer\GAL4^ppk.PU

(Yang et al., 2014)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^GMR.PF

(Yuan et al., 2011)

abnormal oviposition | heat sensitive, with Scer\FLP1^{FLPo.oc.Tag:NLS(SV40-largeT)}, Scer\GAL4^dsx.KI, Scer\GAL80^FRT.αTub84B

(Kimura et al., 2015)

abnormal oviposition | heat sensitive, with Scer\GAL4^dsx.KI

(Kimura et al., 2015)

abnormal sleep | heat sensitive, with Scer\GAL4^NPF.1

(Chung et al., 2017)

abnormal sleep | heat sensitive, with Scer\GAL4^NPF.1, Scer\GAL80^cry.PS

(Chung et al., 2017)

abnormal sleep | heat sensitive, with Scer\GAL4^NPFR.6.6

(Chung et al., 2017)

abnormal sleep | heat sensitive, with Scer\GAL4^Tk.PS

(Chung et al., 2017)

abnormal starvation stress response | heat sensitive, with Scer\GAL4^NPF.1

(Chung et al., 2017)

increased sleep | adult stage, with Scer\GAL4^GMR23E10

(Gong et al., 2021)

Phenotype Manifest In

dendrite | third instar larval stage, with Scer\GAL4^GMR.PF

(Yuan et al., 2011)

larval ventral multidendritic neuron vdaB | somatic clone, with Scer\GAL4^ppk.PU

(Yang et al., 2014)

LNv neuron | third instar larval stage, with Scer\GAL4^GMR.PF

(Yuan et al., 2011)

Detailed Description

Statement

Reference

Activation of Tdc-positive neurons by expressing TrpA1^Scer\UAS.cUa under the control of Scer\GAL4^Tdc2.PC in virgin females at the permissive temperature induces post-mating behaviors. These include reduced levels of copulation within one hour, increased rejection behaviors (ovipositor extrusion) and increased egg laying.

(Rezával et al., 2014)

Single vdaB class IV dendritic arborization neuron cells expressing TrpA1^Scer\UAS.cUa under the control of Scer\GAL4^ppk.PU at 30[o]C show a dorsal shift in the position of their presynaptic terminals compared to controls. The topography of the presynaptic terminals of v'ada class IV da neurons is unaffected if they express TrpA1^Scer\UAS.cUa under the control of Scer\GAL4^ppk.PU as single cell clones at 30[o]C.

(Yang et al., 2014)

Expression of TrpA1^Scer\UAS.cUa under the control of Scer\GAL4^GMR.PF at 29[o]C eliminates the difference in LN(v) dendrite length that is normally seen in constant darkness versus constant light conditions in wild-type larvae.

(Yuan et al., 2011)

Overexpression of TrpA1^Scer\UAS.cUa in class I dendritic arborization neurons (under the control of Scer\GAL4^21-7) confers allyl isothiocyanate sensitivity but not a response to light on these neurons.

(Xiang et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^Dsk-2A-GAL4, TrpA1^UAS.cUa has abnormal copulation phenotype, suppressible by CCKLR-17D3^attP/CCKLR-17D3^attP

(Wang et al., 2022)

Scer\GAL4^GMR71G01, TrpA1^UAS.cUa has abnormal copulation phenotype, suppressible by Dsk^attP/Dsk^attP

(Wang et al., 2022)

Scer\GAL4^GMR23E10, TrpA1^UAS.cUa has increased sleep | adult stage phenotype, suppressible by Iswi^{QUAS.HMS00628}/Ncra\QF^{elav-C155-QF2}

(Gong et al., 2021)

Scer\GAL4^NPF.1, TrpA1^UAS.cUa has abnormal sleep | heat sensitive phenotype, suppressible | partially by NPF^KK111846, Scer\GAL4^NPF.1

(Chung et al., 2017)

NOT suppressed by

Statement

Reference

Scer\GAL4^NPF.1, TrpA1^UAS.cUa has abnormal sleep | heat sensitive phenotype, non-suppressible by NPF^UAS.cWa, Scer\GAL4^NPF.1

(Chung et al., 2017)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

TrpA1^UAS.cUa rescues TrpA1¹

(Peng et al., 2016)

TrpA1^UAS.cUa/Scer\GAL4^TrpA1.PR rescues TrpA1¹

(Kohno et al., 2010)

Comments

Expression of TrpA1^Scer\UAS.cUa under the control of Scer\GAL4^TrpA1.PU rescues the increased temperature preference seen in TrpA1¹ mutant flies.

Expression of TrpA1^Scer\UAS.cUa under the control of Scer\GAL4^ppk.1.9 rescues the delayed initiation of escape behavior in response to touch with a 46[o]C probe seen in TrpA1¹ mutant larvae.

(Peng et al., 2016)

Expression of TrpA1^Scer\UAS.cUa under the control of Scer\GAL4^TrpA1.PR restores the aversive response of TrpA1¹ mutant flies to allyl isothiocyanate.

(Kohno et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

TrpA1^Scer\UAS.cUa

TrpA1^UAS.cUa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Unknown

Secondary FlyBase IDs

References (51)

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