FlyBase Allele Report: Dmel\NPF[KK111846]

General Information

Symbol

Dmel\NPF^KK111846

Species

D. melanogaster

Name

FlyBase ID

FBal0237708

Feature type

allele

Associated gene

Dmel\NPF

Associated Insertion(s)

Carried in Construct

P{KK111846}

Also Known As

UAS-NPF-RNAi, UAS-Npf-IR

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK111846}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK111846

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior, with Scer\FLP1^fru, Scer\GAL4^NPF.1, Scer\GAL80^αTub84B.PG

(Liu et al., 2019)

abnormal courtship behavior, with Scer\GAL4^elav.PLu

(Liu et al., 2019)

abnormal courtship behavior, with Scer\GAL4^NP0021

(Liu et al., 2019)

abnormal courtship behavior, with Scer\GAL4^NPF.1

(Liu et al., 2019)

abnormal mating behavior | mated female, with Scer\GAL4^nSyb.PU

(Lee et al., 2015)

abnormal neurophysiology | adult stage, with Scer\GAL4^386Y

(Lee et al., 2017)

abnormal neurophysiology | adult stage, with Scer\GAL4^Or22a.7.717

(Lee et al., 2017)

abnormal neurophysiology | adult stage, with Scer\GAL4^Orco.PK

(Lee et al., 2017)

abnormal starvation stress response | adult stage, with Scer\GAL4^Tk.PS

(Yoshinari et al., 2021)

decreased cell number | adult stage, with Scer\GAL4^Tk.PS

(Yoshinari et al., 2021)

increased rate of feeding behavior | adult stage, with Scer\GAL4^Tk.PS

(Yoshinari et al., 2021)

Phenotype Manifest In

adult enteroendocrine cell | decreased number, with Scer\GAL4^Tk.PS

(Yoshinari et al., 2021)

adult olfactory receptor neuron Or22, with Scer\GAL4^386Y

(Lee et al., 2017)

adult olfactory receptor neuron Or22, with Scer\GAL4^Or22a.7.717

(Lee et al., 2017)

adult olfactory receptor neuron Or22, with Scer\GAL4^Orco.PK

(Lee et al., 2017)

Detailed Description

Statement

Reference

Expression of NPF^KK111846 under the control of Scer\GAL4^elav.PLu, Scer\GAL4^NP0021 or Scer\GAL4^NPF.1 in males significantly increases courtship towards males, compared to controls; male-male courtship is also increased when expression is restricted to a smaller subset of Scer\GAL4^NPF.1 cells using Scer\GAL80^αTub84B.PG and Scer\FLP1^fru, but not Scer\GAL80^FRT.αTub84B and Scer\FLP1^fru.

(Liu et al., 2019)

The expression of NPF^KK111846 under the control of Scer\GAL4^386Y, Scer\GAL4^NPF.1, Scer\GAL4^Or22a.7.717 or Scer\GAL4^Orco.PK leads to a significant decrease in the frequency of odor-evoked electrophysiological spikes (in response to ethyl butyrate) by adult ab3A neurons, as compared to controls; the Scer\GAL4^NPF.1-driven expression does significantly affect the spontaneous electrophysiological activity of ab3A neurons, nor the ethyl butyrate-evoked electrophysiological activity of adult ab2A and ab1A/B neurons, as compared to controls.

(Lee et al., 2017)

Expression of NPF^KK111846 under the control of Scer\GAL4^nSyb.PU (in combination with UAS-Dicer2 to increase RNAi efficiency) results in a significant increase in the latency to sperm ejection in females after the end of copulation.

(Lee et al., 2015)

Flies expressing NPF^KK111846 either pan-neuronally under the control of Scer\GAL4^elav.PU or in peptidergic neurons under the control of Scer\GAL4^dimm-929 do not show any change in sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503). As in wild type flies, courtship defects are seen in response to (S,Z,Z)-CH503.

(Shankar et al., 2015)

Knockdown of npf through expression of npf^KK111846 under the control of Scer\GAL4^tim.Scer\UAS does not affect LD behaviour.

(Hermann et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

NPF^KK111846, Scer\GAL4^Tk.PS has abnormal starvation stress response | adult stage phenotype, non-enhanceable by Akh^A/Akh[+]

(Yoshinari et al., 2021)

NOT suppressed by

Statement

Reference

NPF^KK111846, Scer\GAL4^Tk.PS has abnormal starvation stress response | adult stage phenotype, non-suppressible by Akh^A/Akh[+]

(Yoshinari et al., 2021)

Suppressor of

Statement

Reference

NPF^KK111846, Scer\GAL4^NPF.1 is a suppressor | partially of abnormal sleep | heat sensitive phenotype of Scer\GAL4^NPF.1, TrpA1^UAS.cUa

(Chung et al., 2017)

Other

Statement

Reference

Akh^A/Akh^attP, NPF^KK111846, Scer\GAL4^Tk.PS has abnormal starvation stress response | adult stage phenotype

(Yoshinari et al., 2021)

NPF^KK111846, Pdf^GD299, Scer\GAL4^tim.UAS has abnormal circadian behavior phenotype

(Hermann et al., 2012)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of NPF^KK111846 reduces the decreases in sleep amount seen in Scer\GAL4^NPF.1>TrpA1^Scer\UAS.cUa flies at 29[o]C compared to controls; total sleep in flies with knockdown is still less than that seen in controls.

(Chung et al., 2017)

In LD 12:12 , the evening activity peak of npf^KK111846/Pdf^GD299 knockdown flies (under the control of Scer\GAL4^tim.Scer\UAS) is different from Pdf^GD299-knockdown flies: it appears broader and flatter.

(Hermann et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v108772

P{KK111846}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

NPF^KK111846

npf^KK111846

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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