FlyBase Allele Report: Scer\GAL4[386Y]

A FLP/FRT intersectional strategy shows expression of EcollexA^{TrpA1-AB-lexA} in larval TrpA1 BCM, BCP, BLA, BLP and VAL neurons when combined with ScerGAL4^386Y.

(Luo et al., 2017)

Expression is observed in the third instar larval midgut. The highest number of GFP-expressing cells was found in the anterior midgut and in the anterior-most part of the posterior midgut. The number of GFP-expressing cells was intermediate in the anterior and posterior part of the posterior midgut, and lowest in the rest of the midgut.

(Reiher et al., 2011)

ScerGAL4^amon.PR is expressed in several larval peptidergic neurons of the brain including the CC-MS, SP3 and in the Tv and Va neurons of the ventral nerve cord.

(Wegener et al., 2011)

Expression is observed in a broad pattern that includes several peptidergic neurons.

(Wu et al., 2003)

Marker for

peptidergic neuron

(Wu et al., 2003)

Reflects expression of

amon

(Bloomington Drosophila Stock Center, 2017, Reiher et al., 2011)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | larval stage, with NPF^UAS.cWa

(Wu et al., 2003)

abnormal developmental rate | conditional, with NPF^UAS.cWa

(Wu et al., 2003)

abnormal feeding behavior | larval stage, with NPF^UAS.cWa

(Wu et al., 2003)

abnormal neurophysiology | adult stage, with CG42260^KK111286

(Lee et al., 2020)

abnormal neurophysiology | adult stage, with NPF^KK111846

(Lee et al., 2017)

abnormal pain response | larval stage, with TrpA1^JF02461

(Luo et al., 2017)

abnormal size | pupal stage, with Alk^{act.UAS.Tag:HA}

(Gouzi et al., 2011)

abnormal size | pupal stage, with Alk^{EC.UAS.Tag:MYC}

(Gouzi et al., 2011)

abnormal size | pupal stage, with Alk^GD42

(Gouzi et al., 2011)

abnormal size | pupal stage, with Alk^UAS.cLa

(Gouzi et al., 2011)

abnormal size | pupal stage, with jeb^UAS.cVa

(Gouzi et al., 2011)

abnormal sleep, with Alk^GD42

(Bai and Sehgal, 2015)

abnormal smell perception | adult stage, with CG42260^KK111286

(Lee et al., 2020)

chemical sensitive, with NPF^UAS.cWa

(Wen et al., 2005)

lethal - all die before end of larval stage, with Leash^d02595

(Zhao et al., 2008)

lethal - all die before end of P-stage, with EY13010^EY13010

(Zhao et al., 2008)

lethal - all die before end of P-stage, with l(3)L0539^EP3523

(Zhao et al., 2008)

partially lethal - majority die, with lark^UAS.Tag:HA

(Schroeder et al., 2003)

short lived | female, with sgg^UAS.cBa

(Trostnikov et al., 2019)

short lived | male, with sgg^UAS.cBa

(Trostnikov et al., 2019)

some die during larval stage, with Leash^d02595

(Zhao et al., 2008)

some die during pharate adult stage, with EY13010^EY13010

(Zhao et al., 2008)

some die during pharate adult stage, with l(3)L0539^EP3523

(Zhao et al., 2008)

some die during pharate adult stage, with lark^UAS.Tag:HA

(Schroeder et al., 2003)

viable, with Sec10^RNAi.UAS

(Andrews et al., 2002)

visible, with lark^UAS.Tag:HA

(Schroeder et al., 2003)

visible | adult stage, with Rab2^KK107630

(Lund et al., 2021)

Phenotype Manifest In

adult cuticle, with lark^UAS.Tag:HA

(Schroeder et al., 2003)

adult olfactory receptor neuron Or22, with CG42260^KK111286

(Lee et al., 2020)

adult olfactory receptor neuron Or22, with NPF^KK111846

(Lee et al., 2017)

wing, with lark^UAS.Tag:HA

(Schroeder et al., 2003)

wing, with Rab2^KK107630

(Lund et al., 2021)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Alk^{EC.UAS.Tag:MYC}/Scer\GAL4^386Y is a suppressor | partially of abnormal size | recessive | pupal stage phenotype of Nf1^E2

(Gouzi et al., 2011)

Alk^GD42/Scer\GAL4^386Y is a suppressor | partially of abnormal size | recessive | larval stage phenotype of Nf1^E2

(Gouzi et al., 2011)

NOT Suppressor of

Statement

Reference

Actβ^CM.UAS/Scer\GAL4^386Y is a non-suppressor of partially lethal - majority die | larval stage phenotype of daw^aact1

(Gesualdi and Haerry, 2007)

babo^Q302D.UAS/Scer\GAL4^386Y is a non-suppressor of partially lethal - majority die | larval stage phenotype of daw^aact1

(Gesualdi and Haerry, 2007)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Alk^UAS.cLa/Scer\GAL4^386Y rescues Alk^ts

(Bai and Sehgal, 2015)

Scer\GAL4^386Y/daw^UAS.cGa rescues daw^aact1

(Gesualdi and Haerry, 2007)

Scer\GAL4^dimm-929, Scer\GAL4^386Y, Phm^UAS.cTA rescues Phm^k07623/Phm^P(29)

(Taghert et al., 2001)

Partially rescues

Scer\GAL4^386Y/RanBPM^S.UAS partially rescues RanBPM^k05201

(Scantlebury et al., 2010)

Nf1^UAS.cWa/Scer\GAL4^386Y partially rescues Nf1^unspecified

(Walker et al., 2006)

Scer\GAL4^386Y/Phm^UAS.cTA partially rescues Phm^k07623/Phm^P(29)

(Taghert et al., 2001)

Fails to rescue

Scer\GAL4^386Y/Ns3^UAS.Venus fails to rescue Ns3^G0431

(Kaplan et al., 2008)

Arc1^UAS.cMb/Scer\GAL4^386Y fails to rescue Arc1^esm18

(Mattaliano et al., 2007)

sbb^UAS.cYa/Scer\GAL4^386Y fails to rescue sbb⁰³⁴³²

(Suster et al., 2004)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

25410

w^*; P{GawB}386Y

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^386Y

Name Synonyms

Secondary FlyBase IDs

References (48)

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