FB2024_03 , released June 25, 2024
Allele: Dmel\ActβCM.UAS
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General Information
Symbol
Dmel\ActβCM.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0144478
Feature type
allele
Associated gene
Dmel\Actβ
Associated Insertion(s)
Carried in Construct
P{UAS-activin-β.CM}
Also Known As
UAS-CMdActivin
Key Links
Transgenic product class
missense_variant
(Zheng et al., 2003)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Zheng et al., 2003)
Mutagen
in vitro construct
(Zheng et al., 2003)
Progenitor genotype
Carried in construct
P{UAS-activin-β.CM}
Cytology
Description

The three Arg residues immediately upstream of the putative processing site of Actβ (residues 563-565) are mutated to Ala, creating a cleavage mutant. This is driven by UASt sequences.

(Zheng et al., 2003)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Actβ
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Ubiquitous expression of two copies of ActβCM.Scer\UAS with Scer\GAL4da.G32 results in smaller animals with wings that are on average 20% smaller than those of wild-type controls.

      Expression of ActβCM.Scer\UAS under the control of Scer\GAL4A9 increases the average wing size to 134% that of wild-type, although no obvious pattern defects are observed.

      (Gesualdi and Haerry, 2007)

      Expression of ActβCM.Scer\UAS specifically in the developing retina under the control of Scer\GAL4GMR.PF results in R7 tiling defects.

      (Ting et al., 2007)

      When activin-βCM.Scer\UAS is driven by Scer\GAL4αTub84B.PL CNS development is retarded, poor development is seen in the optic lobe. When driven by Scer\GAL4OK107 CNS development is not retarded. Expression of activin-βCM.Scer\UAS when driven by Scer\GAL4hs.PB and heat shocked around the mid-third instar stage blocks optic lobe development.

      (Zheng et al., 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Suppressor of
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      ActβCM.UAS, Scer\GAL4GMR.PF has photoreceptor cell R7 | somatic clone phenotype, enhanceable by sevV1

      (Ting et al., 2007)
      Enhancer of
      Statement
      Reference

      Scer\GAL4A9/ActβCM.UAS is an enhancer of wing vein phenotype of SmoxUAS.cGa

      (Gesualdi and Haerry, 2007)
      Suppressor of
      Statement
      Reference

      Scer\GAL4A9/ActβCM.UAS is a suppressor of wing phenotype of MadUAS.cNa

      (Gesualdi and Haerry, 2007)

      Scer\GAL4A9/ActβCM.UAS is a suppressor of wing vein phenotype of MadUAS.cNa

      (Gesualdi and Haerry, 2007)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of ActβCM.Scer\UAS and MadScer\UAS.cNa, both under the control of Scer\GAL4A9 dramatically enhances the ectopic wing vein formation seen in MadScer\UAS.cNa mutants alone.

      Co-expression of ActβCM.Scer\UAS and SmoxScer\UAS.cGa, both under the control of Scer\GAL4A9 results in mild levels of ectopic wing vein formation.

      Expression of ActβCM.Scer\UAS in a dawaact1 background under the control of the neurosecretory driver Scer\GAL4386Y fails to suppress dawaact1 lethality.

      (Gesualdi and Haerry, 2007)

      Although modest, the tiling phenotype found in ActβCM.Scer\UAS mutants is considerably enhanced when neighboring R7s are removed. Approximately 36% of mutant R7s extend laterally into unoccupied columns.

      (Ting et al., 2007)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      ActβCM.Scer\UAS
      ActβCM.UAS
      activin-βCM.Scer\UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (5)