FlyBase Allele Report: Dmel\Alk[GD42]

Using Scer\GAL80^Mef2.mb247 to exclude Alk^GD42 expression from mushroom bodies eliminates the sleep increase induced by Scer\GAL4^386Y or Scer\GAL4^30Y, and in the case of Scer\GAL4^30Y even significantly decreasing total sleep compared to wild type; the decrease in sleep seen with Scer\GAL4^c309 is further significantly decreased.

(Bai and Sehgal, 2015)

Pan-neuronal expression of Alk^GD42 under the control of Scer\GAL4^elav-C155, and restricted to the adult stages using Scer\GAL80^ts.αTub84B, causes a reproducible increase in associative olfactory learning performance compared to controls.

Expression of Alk^GD42 under the control of Scer\GAL4^Ras64B.PW, and restricted to the adult stages using Scer\GAL80^ts.αTub84B, causes a reproducible increase in associative olfactory learning performance compared to controls. This increase in performance is seen when flies are trained with 3 conditional stimulus (CS)/ unconditioned stimulus (US) pairings, and a further enhancement in learning ability is seen with 6 pairings. Training with 12 CS/US pairings does not enhance the phenotype any further. The wings of the resulting adult flies are also larger than in wild type, due to increased cell size rather than cell number.

Expression of Alk^GD42 under the control of Scer\GAL4^Alk.38, and restricted to the adult stages using Scer\GAL80^ts.αTub84B, causes a reproducible increase in associative olfactory learning performance compared to controls. This increase in performance is seen when flies are trained with 3 conditional stimulus (CS)/ unconditioned stimulus (US) pairings.

Expression of Alk^GD42 under the control of either Scer\GAL4^Mef2.247 or Scer\GAL4^c747, and restricted to the adult stages using Scer\GAL80^ts.αTub84B, does not affect associative olfactory learning.

Pan-neuronal expression of Alk^GD42 under the control of Scer\GAL4^elav-C155 throughout development yields pupae that are 10-15% larger than controls. The resulting adults are viable and normally patterned.

Expression of Alk^GD42 under the control of Scer\GAL4^Ras64B.PW throughout development yields pupae that are 10-15% larger than controls.

Expression of Alk^GD42 under the control of Scer\GAL4^Alk.38 throughout development yields pupae that are 10-15% larger than controls.

Expression of Alk^GD42 under the control of Scer\GAL4^386Y throughout development yields pupae that are 10-15% larger than controls.

Expression of Alk^GD42 under the control of Scer\GAL4^Ilp2.PR throughout development yields pupae that are a similar size to controls.

(Gouzi et al., 2011)

Adults expressing Alk^GD42 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Alk^GD42, Scer\GAL4^nSyb.PS has abnormal sleep phenotype, suppressible by Nf1^P2/Nf1^P1

(Bai and Sehgal, 2015)

NOT Enhancer of

Statement

Reference

Scer\GAL80^ts.αTub84B, Alk^GD42, Scer\GAL4^da.PU is a non-enhancer of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4^da.PU, Scer\GAL80^ts.αTub84B, fzr^RNAi.UAS.WIZ

(Neuert et al., 2017)

Suppressor of

Statement

Reference

Alk^GD42/Scer\GAL4^Ras64B.PW is a suppressor of abnormal learning | adult stage phenotype of Nf1^E1/Nf1^E2

(Gouzi et al., 2011)

Alk^GD42/Scer\GAL4^Alk.38 is a suppressor of abnormal learning | adult stage phenotype of Nf1^E1/Nf1^E2

(Gouzi et al., 2011)

Scer\GAL4^elav-C155/Alk^GD42 is a suppressor | partially of abnormal size | recessive | larval stage phenotype of Nf1^E2

(Gouzi et al., 2011)

Alk^GD42/Scer\GAL4^Ras64B.PW is a suppressor | partially of abnormal size | recessive | larval stage phenotype of Nf1^E2

(Gouzi et al., 2011)

Alk^GD42/Scer\GAL4^Ras64B.PW is a suppressor | partially of decreased cell size | adult stage phenotype of Nf1^E2

(Gouzi et al., 2011)

Alk^GD42/Scer\GAL4^386Y is a suppressor | partially of abnormal size | recessive | larval stage phenotype of Nf1^E2

(Gouzi et al., 2011)

Scer\GAL4^elav-C155/Alk^GD42 is a suppressor of abnormal learning | adult stage phenotype of Nf1^E1/Nf1^E2

(Gouzi et al., 2011)

NOT Suppressor of

Statement

Reference

Scer\GAL80^ts.αTub84B, Alk^GD42, Scer\GAL4^da.PU is a non-suppressor of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4^da.PU, Scer\GAL80^ts.αTub84B, fzr^RNAi.UAS.WIZ

(Neuert et al., 2017)

Scer\GAL4^ey-OK107/Alk^GD42 is a non-suppressor of abnormal size | recessive | larval stage phenotype of Nf1^E2

(Gouzi et al., 2011)

Scer\GAL4^CCAP.PP/Alk^GD42 is a non-suppressor of abnormal size | recessive | larval stage phenotype of Nf1^E2

(Gouzi et al., 2011)

Scer\GAL4^c747/Alk^GD42 is a non-suppressor of abnormal learning | adult stage phenotype of Nf1^E1/Nf1^E2

(Gouzi et al., 2011)

Phenotype Manifest In

Suppressor of

Statement

Reference

Alk^GD42/Scer\GAL4^Ras64B.PW is a suppressor | partially of wing phenotype of Nf1^E2

(Gouzi et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Nf1^P1/Nf1^P2 suppresses the long sleep phenotype seen in flies with Alk^GD42 driven by Scer\GAL4^nSyb.PS (at 25[o]C in female flies).

(Bai and Sehgal, 2015)

Expression of Alk^GD42 under the control of Scer\GAL4^elav-C155 rescues the small pupae phenotype seen in Nf1^E2 homozygotes. The pupae seen are close to the size of controls. Rescue is also seen when Alk^{EC.Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^Ras64B.PW but the rescue is not as efficient. The increase in size is due to increased cell size rather than increased cell number. Expression of Alk^GD42 under the control of Scer\GAL4^386Y also partially rescues the phenotype in Nf1^E2.

Expression of Alk^GD42 under the control of Scer\GAL4^ey-OK107 does not suppress the small pupae phenotype seen in Nf1^E2 mutants.

Expression of Alk^GD42 under the control of Scer\GAL4^Ccap.PP does not suppress the small pupae phenotype seen in Nf1^E2 mutants.

Expression of Alk^GD42 under the control of either the Scer\GAL4^elav-C155, Scer\GAL4^Ras64B.PW or Scer\GAL4^Alk.38 driver, and restricted to the adult stages using Scer\GAL80^ts.αTub84B, rescues the olfactory learning defects seen in Nf1^E2/Nf1^E1 mutants.

Expression of Alk^GD42 under the control of Scer\GAL4^c747, and restricted to the adult stages using Scer\GAL80^ts.αTub84B, fails to rescue the olfactory learning defects seen in Nf1^E2/Nf1^E1 mutants.

(Gouzi et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v11446

w¹¹¹⁸ P{GD42}v11446

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Alk^GD42

(Hertenstein et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (11)

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