FlyBase Allele Report: Scer\GAL4[Ras64B.PW]

General Information

Symbol

Scer\GAL4^Ras64B.PW

Species

S. cerevisiae

Name

Ras oncogene at 64B promoter construct of Walker

FlyBase ID

FBal0197136

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{Ras64B-GAL4.W}

Also Known As

Ras2-Gal4, Ras2GAL4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Walker et al., 2006)

Progenitor genotype

Carried in construct

P{Ras64B-GAL4.W}

Cytology

Description

A 358bp segment of the Ras64B promoter region regulates expression of a GAL4 driver.

(Walker et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

Ras64B

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

embryonic/larval brain | restricted

(Gouzi et al., 2011)

adult stage

adult brain | restricted

(Gouzi et al., 2011)

calyx of adult mushroom body

(Gouzi et al., 2011)

lobe system of mushroom body

(Gouzi et al., 2011)

gnathal ganglion

(Gouzi et al., 2011)

adult antennal lobe

(Gouzi et al., 2011)

Additional Information

Statement

Reference

ScerGAL4^Ras64B.PW drives widespread expression in the third instar larval and adult brain. There is extensive colocalisation with Alk protein, namely in the mushroom body calyx, antennal lobe and subesophageal ganglion. ScerGAL4^Ras64B.PW also labels the lobe system of the mushroom bodies.

(Gouzi et al., 2011)

Marker for

Reflects expression of

Ras64B

(Gouzi et al., 2011)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal learning | adult stage, with Alk^{act.UAS.Tag:HA}, Scer\GAL80^ts.αTub84B

(Gouzi et al., 2011)

abnormal learning | adult stage, with Alk^{EC.UAS.Tag:MYC}, Scer\GAL80^ts.αTub84B

(Gouzi et al., 2011)

abnormal learning | adult stage, with Alk^GD42, Scer\GAL80^ts.αTub84B

(Gouzi et al., 2011)

abnormal learning | adult stage, with Alk^UAS.cLa, Scer\GAL80^ts.αTub84B

(Gouzi et al., 2011)

abnormal learning | adult stage, with Scer\GAL80^ts.αTub84B, jeb^UAS.cVa

(Gouzi et al., 2011)

abnormal size | pupal stage, with Alk^{act.UAS.Tag:HA}

(Gouzi et al., 2011)

abnormal size | pupal stage, with Alk^{EC.UAS.Tag:MYC}

(Gouzi et al., 2011)

abnormal size | pupal stage, with Alk^GD42

(Gouzi et al., 2011)

abnormal size | pupal stage, with Alk^UAS.cLa

(Gouzi et al., 2011)

abnormal size | pupal stage, with jeb^UAS.cVa

(Gouzi et al., 2011)

abnormal size | pupal stage, with rl^Sem.MB.UAS

(Gouzi et al., 2011)

decreased body size | adult stage, with Raf^UAS.F179

(Walker et al., 2006)

decreased body size | adult stage, with Ras64B^Val14.UAS

(Walker et al., 2006)

decreased body size | adult stage, with Ras85D^V12.cGa.UAS

(Walker et al., 2006)

decreased cell size | adult stage, with Alk^{act.UAS.Tag:HA}

(Gouzi et al., 2011)

decreased cell size | adult stage, with Alk^UAS.cLa

(Gouzi et al., 2011)

increased cell size | adult stage, with Alk^{EC.UAS.Tag:MYC}

(Gouzi et al., 2011)

increased cell size | adult stage, with Alk^GD42

(Gouzi et al., 2011)

lethal - all die before end of pupal stage | heat sensitive, with Ras64B^Val14.UAS

(Walker et al., 2006)

lethal - all die before end of pupal stage | heat sensitive, with Ras85D^V12.cGa.UAS

(Walker et al., 2006)

partially lethal, with Mical^UAS.EGFP, Scer\GAL4^elav-C155

(Prifti et al., 2022)

some die during pupal stage | heat sensitive, with Ras64B^Val14.UAS

(Walker et al., 2006)

some die during pupal stage | heat sensitive, with Ras85D^V12.cGa.UAS

(Walker et al., 2006)

Phenotype Manifest In

wing, with Alk^{act.UAS.Tag:HA}

(Gouzi et al., 2011)

wing, with Alk^{EC.UAS.Tag:MYC}

(Gouzi et al., 2011)

wing, with Alk^GD42

(Gouzi et al., 2011)

wing, with Alk^UAS.cLa

(Gouzi et al., 2011)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Mical^UAS.EGFP, Scer\GAL4^Ras64B.PW, Scer\GAL4^elav-C155 has partially lethal phenotype, non-enhanceable by Hsap\MAPT^{C322A.UAS.2N4R.Tag:FLAG}, Scer\GAL4^Ras64B.PW, Scer\GAL4^elav-C155

(Prifti et al., 2022)

NOT suppressed by

Statement

Reference

Mical^UAS.EGFP, Scer\GAL4^Ras64B.PW, Scer\GAL4^elav-C155 has partially lethal phenotype, non-suppressible by Hsap\MAPT^{C322A.UAS.2N4R.Tag:FLAG}, Scer\GAL4^Ras64B.PW, Scer\GAL4^elav-C155

(Prifti et al., 2022)

NOT Enhancer of

Statement

Reference

Hsap\MAPT^{C322A.UAS.2N4R.Tag:FLAG}, Scer\GAL4^Ras64B.PW, Scer\GAL4^elav-C155 is a non-enhancer of partially lethal phenotype of Mical^UAS.EGFP, Scer\GAL4^Ras64B.PW, Scer\GAL4^elav-C155

(Prifti et al., 2022)

Suppressor of

Statement

Reference

Scer\GAL4^Ras64B.PW/jeb^KK111857 is a suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

Alk^KK102942/Scer\GAL4^Ras64B.PW is a suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

Scer\GAL4^Ras64B.PW/Dsor1^KK102276 is a suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

Scer\GAL4^Ras64B.PW/amx^GD1168 is a suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

cnk^KK109209/Scer\GAL4^Ras64B.PW is a suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

CCKLR-17D1^KK108482/Scer\GAL4^Ras64B.PW is a suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

Dap160^KK102297/Scer\GAL4^Ras64B.PW is a suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

Scer\GAL4^Ras64B.PW/Rab9^KK109041 is a suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

Dcr-2^UAS.cDa, Scer\GAL4^Ras64B.PW, NAAT1^KK112890 is a suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

Scer\GAL4^Ras64B.PW/HERC2^KK100181 is a suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

Scer\GAL4^Ras64B.PW/Hs3st-B^KK108793 is a suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

Alk^GD42/Scer\GAL4^Ras64B.PW is a suppressor | partially of abnormal size | recessive | larval stage phenotype of Nf1^E2

(Gouzi et al., 2011)

Alk^GD42/Scer\GAL4^Ras64B.PW is a suppressor | partially of decreased cell size | adult stage phenotype of Nf1^E2

(Gouzi et al., 2011)

Alk^{EC.UAS.Tag:MYC}/Scer\GAL4^Ras64B.PW is a suppressor of abnormal learning | adult stage phenotype of Nf1^E1/Nf1^E2

(Gouzi et al., 2011)

Alk^GD42/Scer\GAL4^Ras64B.PW is a suppressor of abnormal learning | adult stage phenotype of Nf1^E1/Nf1^E2

(Gouzi et al., 2011)

Alk^{EC.UAS.Tag:MYC}/Scer\GAL4^Ras64B.PW is a suppressor | partially of abnormal size | recessive | pupal stage phenotype of Nf1^E2

(Gouzi et al., 2011)

vap^UAS.Tag:MYC/Scer\GAL4^Ras64B.PW is a suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2006)

NOT Suppressor of

Statement

Reference

Hsap\MAPT^{C322A.UAS.2N4R.Tag:FLAG}, Scer\GAL4^Ras64B.PW, Scer\GAL4^elav-C155 is a non-suppressor of partially lethal phenotype of Mical^UAS.EGFP, Scer\GAL4^Ras64B.PW, Scer\GAL4^elav-C155

(Prifti et al., 2022)

CCKLR-17D3^GD3431/Scer\GAL4^Ras64B.PW is a non-suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

Scer\GAL4^Ras64B.PW/dnc^KK108541 is a non-suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

Scer\GAL4^Ras64B.PW/Mmus\Prkaca^act.2xUAS is a non-suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

Scer\GAL4^Ras64B.PW/Mmus\Prkaca^act.3xUAS is a non-suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

Scer\GAL4^Ras64B.PW/RasGAP1^UAS.cWa is a non-suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2006)

Phenotype Manifest In

Suppressor of

Statement

Reference

Alk^GD42/Scer\GAL4^Ras64B.PW is a suppressor | partially of wing phenotype of Nf1^E2

(Gouzi et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Nf1^UAS.cWa/Scer\GAL4^Ras64B.PW rescues Nf1^E2

(Walker et al., 2013)

Nf1^UAS.cWa, Scer\GAL4^Ras64B.PW, Scer\GAL80^ts.αTub84B rescues Nf1^E1/Nf1^E2

(Gouzi et al., 2011)

Nf1^UAS.cWa/Scer\GAL4^Ras64B.PW rescues Nf1^E2

(Walker et al., 2006)

Nf1^{GRD.UAS.Tag:HA}/Scer\GAL4^Ras64B.PW rescues Nf1^E2

(Walker et al., 2006)

Fails to rescue

Scer\GAL4^Ras64B.PW/Nf1^{R1320P.GRD.UAS.Tag:HA} fails to rescue Nf1^E2

(Walker et al., 2006)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^Ras64B.PW

Name Synonyms

Ras oncogene at 64B promoter construct of Walker

Secondary FlyBase IDs

References (10)

Report Sections

Open Close