FlyBase Allele Report: Dmel\vap[UAS.Tag:MYC]

General Information

Symbol

Dmel\vap^UAS.Tag:MYC

Species

D. melanogaster

Name

FlyBase ID

FBal0097181

Feature type

allele

Associated gene

Dmel\vap

Associated Insertion(s)

Carried in Construct

P{UAS-RasGAP.Myc}

Also Known As

P[UAS-RasGAP]

Key Links

Transgenic product class

cDNA

(Feldmann et al., 1999)

wild_type

(Feldmann et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Feldmann et al., 1999)

wild_type

(Feldmann et al., 1999)

Mutagen

in vitro construct

(Feldmann et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-RasGAP.Myc}

Cytology

Description

A full length vap cDNA tagged with vap is expressed under the control of UASt regulatory sequences.

(Feldmann et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

vap

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates neurofibromatosis

modeled by Nf1^E2

(Walker et al., 2006)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell size | larval stage | somatic clone, with Scer\GAL4^Act5C.PU

(Bourouis et al., 2019)

long lived, with Scer\GAL4^elav.PU, vap^KS67

(Bourouis et al., 2019)

visible, with Scer\GAL4^Bx-MS1096

(Feldmann et al., 1999)

Phenotype Manifest In

autophagosome | larval stage | somatic clone, with Scer\GAL4^Act5C.PU

(Bourouis et al., 2019)

embryonic/larval fat body | larval stage | somatic clone, with Scer\GAL4^Act5C.PU

(Bourouis et al., 2019)

lysosome | larval stage | somatic clone, with Scer\GAL4^Act5C.PU

(Bourouis et al., 2019)

wing, with Scer\GAL4^Bx-MS1096

(Feldmann et al., 1999)

Detailed Description

Statement

Reference

Clonal expression of vap^UAS.Tag:MYC under the control of Scer\GAL4^Act5C.PU in the larval fat body prevents the starvation-induced formation of lysosomes (LysoTracker) and autophagosomes ( GFP:Atg8a ) observed in controls; in well fed individuals, the clonal cells are slightly but significantly larger than in controls.

The sensitivity of vap^KS67 homozygous adults to acute food deprivation is fully rescued by the expression of vap^UAS.Tag:MYC under the control of Scer\GAL4^elav.PU, even leading to resistance to food deprivation, as compared to controls.

(Bourouis et al., 2019)

Expression of vap^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ey.PH or Scer\GAL4^GMR.PF does not cause a detectable phenotype. Expression of vap^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^hs.2sev does not affect photoreceptor function. Expression of vap^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Bx-MS1096 results in wings which are smaller than normal and have an upward curvature. The number of hairs on the dorsal margin of the wing between veins L3 and L4 is reduced compared to wild type, while the cell diameter along the wing margin is unaffected. The longitudinal wing veins are not disrupted.

(Feldmann et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/vap^UAS.Tag:MYC is a suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2006)

vap^UAS.Tag:MYC/Scer\GAL4^Ras64B.PW is a suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2006)

vap^UAS.Tag:MYC/Scer\GAL4^Bx-MS1096 is a suppressor | partially of visible phenotype of Scer\GAL4^Bx-MS1096, htl::tor^4021.UAS

(Woodcock and Hughes, 2004)

vap^UAS.Tag:MYC/Scer\GAL4^Bx-MS1096 is a suppressor of visible phenotype of Scer\GAL4^Bx-MS1096, btl::tor^4021.UAS

(Feldmann et al., 1999)

vap^UAS.Tag:MYC/Scer\GAL4^Bx-MS1096 is a suppressor of visible phenotype of Scer\GAL4^Bx-MS1096, htl::tor^4021.UAS

(Feldmann et al., 1999)

NOT Suppressor of

Statement

Reference

vap^UAS.Tag:MYC/Scer\GAL4^repo is a non-suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2006)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^Bx-MS1096, vap^UAS.Tag:MYC has wing phenotype, non-enhanceable by Ras85D^e2F

(Feldmann et al., 1999)

Suppressor of

Statement

Reference

vap^UAS.Tag:MYC/Scer\GAL4^Bx-MS1096 is a suppressor | partially of wing vein | ectopic phenotype of Scer\GAL4^Bx-MS1096, htl::tor^4021.UAS

(Woodcock and Hughes, 2004)

vap^UAS.Tag:MYC/Scer\GAL4^Bx-MS1096 is a suppressor of wing phenotype of Scer\GAL4^Bx-MS1096, btl::tor^4021.UAS

(Feldmann et al., 1999)

vap^UAS.Tag:MYC/Scer\GAL4^Bx-MS1096 is a suppressor of wing vein phenotype of Scer\GAL4^Bx-MS1096, btl::tor^4021.UAS

(Feldmann et al., 1999)

vap^UAS.Tag:MYC/Scer\GAL4^Bx-MS1096 is a suppressor of wing phenotype of Scer\GAL4^Bx-MS1096, htl::tor^4021.UAS

(Feldmann et al., 1999)

vap^UAS.Tag:MYC/Scer\GAL4^Bx-MS1096 is a suppressor of wing vein phenotype of Scer\GAL4^Bx-MS1096, htl::tor^4021.UAS

(Feldmann et al., 1999)

NOT Suppressor of

Statement

Reference

vap^UAS.Tag:MYC/Scer\GAL4^hs.2sev is a non-suppressor of phenotype of sev^S11.Tag:MYC

(Feldmann et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Does not suppress the sev^{S11.T:Hsap\MYC} phenotype when expressed under the control of Scer\GAL4^hs.2sev. The wing phenotype caused by expression of vap^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Bx-MS1096 is not enhanced by one copy of Ras85D^e2F. Suppresses the wing phenotype caused by btl::tor^{4021.Scer\UAS} or htl::tor^{4021.Scer\UAS} expressed under the control of Scer\GAL4^Bx-MS1096.