FlyBase Allele Report: Dmel\Alk[EC.UAS.Tag:MYC]

General Information

Symbol

Dmel\Alk^{EC.UAS.Tag:MYC}

Species

D. melanogaster

Name

FlyBase ID

FBal0194692

Feature type

allele

Associated gene

Dmel\Alk

Associated Insertion(s)

Carried in Construct

P{UAS-Alk.EC.MYC}

Also Known As

UAS-Alk^DN, UAS-AlkEC, UAS-Alk.EC.MYC

Key Links

Transgenic product class

dominant_negative_variant

(Bazigou et al., 2007)

inframe_deletion

(Bazigou et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Bazigou et al., 2007)

inframe_deletion

(Bazigou et al., 2007)

Mutagen

in vitro construct

(Bazigou et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-Alk.EC.MYC}

Cytology

Description

UASt regulatory regions drive expression of the extracellular and transmembrane domains of Alk, which is tagged at the C-terminus with a Tag:MYC tag.

(Bazigou et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Alk

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates neurofibromatosis

modeled by Nf1^E2

(Gouzi et al., 2011)

modeled by Nf1^JF01866

(Georganta et al., 2021)

modeled by Nf1^P1, Nf1^P2

(Moscato et al., 2020)

ameliorates neurofibromatosis 1

modeled by Nf1^E1, Nf1^E2

(Durkin et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate, with Scer\GAL4^phtm.PO

(Pan and O'Connor, 2021)

abnormal learning | adult stage, with Scer\GAL4^Alk.38, Scer\GAL80^ts.αTub84B

(Gouzi et al., 2011)

abnormal learning | adult stage, with Scer\GAL4^elav-C155, Scer\GAL80^ts.αTub84B

(Gouzi et al., 2011)

abnormal learning | adult stage, with Scer\GAL4^Ras64B.PW, Scer\GAL80^ts.αTub84B

(Gouzi et al., 2011)

abnormal neuroanatomy | somatic clone, with Scer\GAL4^Act5C.PI

(Bazigou et al., 2007)

abnormal size | pupal stage, with Scer\GAL4^386Y

(Gouzi et al., 2011)

abnormal size | pupal stage, with Scer\GAL4^Alk.38

(Gouzi et al., 2011)

abnormal size | pupal stage, with Scer\GAL4^elav-C155

(Gouzi et al., 2011)

abnormal size | pupal stage, with Scer\GAL4^Ras64B.PW

(Gouzi et al., 2011)

increased body size | P-stage, with Scer\GAL4^elav-C155

(Pfeifer et al., 2022)

increased cell size | adult stage, with Scer\GAL4^Ras64B.PW

(Gouzi et al., 2011)

partially lethal, with Scer\GAL4^phtm.PO

(Pan and O'Connor, 2021)

viable, with Scer\GAL4^elav-C155

(Gouzi et al., 2011)

Phenotype Manifest In

axon & photoreceptor cell R8 | somatic clone, with Scer\GAL4^Act5C.PI

(Bazigou et al., 2007)

pupa, with Scer\GAL4^elav-C155

(Pfeifer et al., 2022)

visceral mesoderm, with Scer\GAL4^twi.PG

(Bazigou et al., 2007)

wing, with Scer\GAL4^Ras64B.PW

(Gouzi et al., 2011)

Detailed Description

Statement

Reference

Pan-neuronal expression of Alk^{EC.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^elav-C155, and restricted to the adult stages using Scer\GAL80^ts.αTub84B, causes a reproducible increase in associative olfactory learning performance compared to controls.

Expression of Alk^{EC.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Ras64B.PW, and restricted to the adult stages using Scer\GAL80^ts.αTub84B, causes a reproducible increase in associative olfactory learning performance compared to controls. This increase in performance is seen when flies are trained with 3 conditional stimulus (CS)/ unconditioned stimulus (US) pairings, and a further enhancement in learning ability is seen with 6 pairings. Training with 12 CS/US pairings does not enhance the phenotype any further. The wings of the resulting adult flies are also larger than in wild type, due to increased cell size rather than cell number.

Expression of Alk^{EC.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Alk.38, and restricted to the adult stages using Scer\GAL80^ts.αTub84B, causes a reproducible increase in associative olfactory learning performance compared to controls. This increase in performance is seen when flies are trained with 3 conditional stimulus (CS)/ unconditioned stimulus (US) pairings.

Expression of Alk^{EC.Scer\UAS.T:Hsap\MYC} under the control of either Scer\GAL4^Mef2.247 or Scer\GAL4^c747, and restricted to the adult stages using Scer\GAL80^ts.αTub84B, does not affect associative olfactory learning.

Pan-neuronal expression of Alk^{EC.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^elav-C155 throughout development yields pupae that are 10-15% larger than controls. The resulting adults are viable and normally patterned.

Expression of Alk^{EC.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Ras64B.PW throughout development yields pupae that are 10-15% larger than controls.

Expression of Alk^{EC.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Alk.38 throughout development yields pupae that are 10-15% larger than controls.

Expression of Alk^{EC.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^386Y throughout development yields pupae that are 10-15% larger than controls.

Expression of Alk^{EC.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Ilp2.PR throughout development yields pupae that are a similar size to controls.

(Gouzi et al., 2011)

The visceral mesoderm is disrupted in stage 14 embryos that express Alk^{EC.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^twi.PG.

R8 axons aberrantly terminate at the distal border of the medulla neuropil in animals that have clones overexpressing Alk^{EC.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Act5C.PI in the visual system. The M3 recipient layer in these animals appear largely devoid of R8 terminals.

(Bazigou et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^elav.PU/Alk^{EC.UAS.Tag:MYC} is a suppressor of decreased sleep | larval stage phenotype of Nf1^E1/Nf1^E2

(Durkin et al., 2023)

Scer\GAL4^elav-C155/Alk^{EC.UAS.Tag:MYC} is a suppressor | partially of abnormal size | recessive | pupal stage phenotype of Nf1^E2

(Gouzi et al., 2011)

Alk^{EC.UAS.Tag:MYC}/Scer\GAL4^Ras64B.PW is a suppressor | partially of abnormal size | recessive | pupal stage phenotype of Nf1^E2

(Gouzi et al., 2011)

Alk^{EC.UAS.Tag:MYC}/Scer\GAL4^386Y is a suppressor | partially of abnormal size | recessive | pupal stage phenotype of Nf1^E2

(Gouzi et al., 2011)

Scer\GAL4^elav-C155/Alk^{EC.UAS.Tag:MYC} is a suppressor of abnormal learning | adult stage phenotype of Nf1^E1/Nf1^E2

(Gouzi et al., 2011)

Alk^{EC.UAS.Tag:MYC}/Scer\GAL4^Ras64B.PW is a suppressor of abnormal learning | adult stage phenotype of Nf1^E1/Nf1^E2

(Gouzi et al., 2011)

Alk^{EC.UAS.Tag:MYC}/Scer\GAL4^Alk.38 is a suppressor of abnormal learning | adult stage phenotype of Nf1^E1/Nf1^E2

(Gouzi et al., 2011)

NOT Suppressor of

Statement

Reference

Scer\GAL4^ey-OK107/Alk^{EC.UAS.Tag:MYC} is a non-suppressor of abnormal size | recessive | larval stage phenotype of Nf1^E2

(Gouzi et al., 2011)

Alk^{EC.UAS.Tag:MYC}/Scer\GAL4^CCAP.PP is a non-suppressor of abnormal size | recessive | larval stage phenotype of Nf1^E2

(Gouzi et al., 2011)

Scer\GAL4^c747/Alk^{EC.UAS.Tag:MYC} is a non-suppressor of abnormal learning | adult stage phenotype of Nf1^E1/Nf1^E2

(Gouzi et al., 2011)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Alk^{EC.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^elav-C155 significantly suppresses the small pupae phenotype seen in Nf1^E2 homozygotes. The pupae seen are close to the size of controls. Rescue is also seen when Alk^{EC.Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^Ras64B.PW or Scer\GAL4^386Y but to a lesser extent.

Expression of Alk^{EC.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ey-OK107 does not suppress the small pupae phenotype seen in Nf1^E2 mutants.

Expression of Alk^{EC.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Ccap.PP does not suppress the small pupae phenotype seen in Nf1^E2 mutants.

Expression of Alk^{EC.Scer\UAS.T:Hsap\MYC} under the control of either the Scer\GAL4^elav-C155, Scer\GAL4^Ras64B.PW or Scer\GAL4^Alk.38 driver, and restricted to the adult stages using Scer\GAL80^ts.αTub84B, rescues the olfactory learning defects seen in Nf1^E2/Nf1^E1 mutants.

Expression of Alk^{EC.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^c747, and restricted to the adult stages using Scer\GAL80^ts.αTub84B, fails to rescue the olfactory learning defects seen in Nf1^E2/Nf1^E1 mutants.

(Gouzi et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Alk^{EC.Scer\UAS.T:Hsap\MYC}

Alk^{EC.UAS.Tag:MYC}

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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