FlyBase Allele Report: Dmel\Nf1[UAS.cWa]

General Information

Symbol

Dmel\Nf1^UAS.cWa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Walker

FlyBase ID

FBal0197118

Feature type

allele

Associated gene

Dmel\Nf1

Associated Insertion(s)

Carried in Construct

P{UAS-Nf1.W}

Also Known As

UAS-dNf1

Key Links

Transgenic product class

wild_type

(Walker et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Walker et al., 2006)

Mutagen

in vitro construct

(Walker et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-Nf1.W}

Cytology

Description

UAS regulatory sequences drive expression of Nf1. The construct includes the shorter Nf1 C-terminus predicted by exon 18b and lacks a 30 amino acid segment predicted by alternatively spliced exon 14.

(Walker et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Nf1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Nf1^UAS.cWa/Scer\GAL4^C23 rescues Nf1^E2

(Walker et al., 2013)

Nf1^UAS.cWa/Scer\GAL4^Ras64B.PW rescues Nf1^E2

(Walker et al., 2013)

Scer\GAL4^Act5C.PU/Nf1^UAS.cWa rescues Nf1^E2

(Walker et al., 2013)

Scer\GAL4^elav.PU/Nf1^UAS.cWa rescues Nf1^E2

(Walker et al., 2013)

Nf1^UAS.cWa/Scer\GAL4^Alk.38 rescues Nf1^E1/Nf1^E2

(Gouzi et al., 2011)

Scer\GAL4^elav-C155, Nf1^UAS.cWa, Scer\GAL80^ts.αTub84B rescues Nf1^E1/Nf1^E2

(Gouzi et al., 2011)

Nf1^UAS.cWa, Scer\GAL4^Ras64B.PW, Scer\GAL80^ts.αTub84B rescues Nf1^E1/Nf1^E2

(Gouzi et al., 2011)

Nf1^UAS.cWa, Scer\GAL4^Alk.38, Scer\GAL80^ts.αTub84B rescues Nf1^E1/Nf1^E2

(Gouzi et al., 2011)

Scer\GAL4^elav-C155, Scer\GAL80^Mef2.mb247, Nf1^UAS.cWa rescues Nf1^E1/Nf1^E2

(Gouzi et al., 2011)

Scer\GAL4^Act5C.PI/Nf1^UAS.cWa rescues Nf1^E2

(Walker et al., 2006)

Nf1^UAS.cWa/Scer\GAL4^C23 rescues Nf1^E2

(Walker et al., 2006)

Scer\GAL4^elav-C155/Nf1^UAS.cWa rescues Nf1^E2

(Walker et al., 2006)

Nf1^UAS.cWa/Scer\GAL4^Ras64B.PW rescues Nf1^E2

(Walker et al., 2006)

Scer\GAL4^Ras85D.PW/Nf1^UAS.cWa rescues Nf1^E2

(Walker et al., 2006)

Partially rescues

Nf1^UAS.cWa/Scer\GAL4^386Y partially rescues Nf1^unspecified

(Walker et al., 2006)

Scer\GAL4^elav-C155/Nf1^UAS.cWa partially rescues Nf1^E1/Nf1^E2

(Walker et al., 2006)

Fails to rescue

Nf1^UAS.cWa/Scer\GAL4^Ret.PW fails to rescue Nf1^E2

(Walker et al., 2013)

Scer\GAL4^Feb36/Nf1^UAS.cWa fails to rescue Nf1^E2

(Walker et al., 2013)

Scer\GAL4^Aug21/Nf1^UAS.cWa fails to rescue Nf1^E2

(Walker et al., 2013)

Scer\GAL4^Akh.PW/Nf1^UAS.cWa fails to rescue Nf1^E2

(Walker et al., 2013)

Scer\GAL4^c747, Nf1^UAS.cWa, Scer\GAL80^ts.αTub84B fails to rescue Nf1^E1/Nf1^E2

(Gouzi et al., 2011)

Scer\GAL4^Mef2.247, Nf1^UAS.cWa, Scer\GAL80^ts.αTub84B fails to rescue Nf1^E1/Nf1^E2

(Gouzi et al., 2011)

Scer\GAL4^ey-OK107, Nf1^UAS.cWa, Scer\GAL80^ts.αTub84B fails to rescue Nf1^E1/Nf1^E2

(Gouzi et al., 2011)

Nf1^UAS.cWa/Scer\GAL4^repo fails to rescue Nf1^unspecified

(Walker et al., 2006)

Nf1^UAS.cWa/Scer\GAL4^phtm.PO fails to rescue Nf1^unspecified

(Walker et al., 2006)

Scer\GAL4^Aug21/Nf1^UAS.cWa fails to rescue Nf1^unspecified

(Walker et al., 2006)

Scer\GAL4^P0206/Nf1^UAS.cWa fails to rescue Nf1^unspecified

(Walker et al., 2006)

Scer\GAL4^Mai369/Nf1^UAS.cWa fails to rescue Nf1^unspecified

(Walker et al., 2006)

Scer\GAL4^{CenB1A-Mai301}/Nf1^UAS.cWa fails to rescue Nf1^unspecified

(Walker et al., 2006)

Nf1^UAS.cWa/Scer\GAL4^Ddc.PL fails to rescue Nf1^unspecified

(Walker et al., 2006)

Nf1^UAS.cWa/Scer\GAL4^ChAT.7.4 fails to rescue Nf1^unspecified

(Walker et al., 2006)

Nf1^UAS.cWa/Scer\GAL4^amn-c651 fails to rescue Nf1^unspecified

(Walker et al., 2006)

Nf1^UAS.cWa/Scer\GAL4^Ilp2.PR fails to rescue Nf1^unspecified

(Walker et al., 2006)

Nf1^UAS.cWa/Scer\GAL4^repo fails to rescue Nf1^E2

(Walker et al., 2006)

Scer\GAL4^en-e16E/Nf1^UAS.cWa fails to rescue Nf1^E1/Nf1^E2

(Walker et al., 2006)

Comments

Expression of Nf1^Scer\UAS.cWa under the control of either Scer\GAL4^Act5C.PU, Scer\GAL4^elav.PU, Scer\GAL4^Ras64B.PW or Scer\GAL4^C23 rescues the reduced pupal length phenotype seen in Nf1^E2 animals, while expression under the control of either Scer\GAL4^Ret.PW, Scer\GAL4^Feb36, Scer\GAL4^Aug21 or Scer\GAL4^Akh.PW does not rescue.

(Walker et al., 2013)

Expression of Nf1^Scer\UAS.cWa under the control of Scer\GAL4^Alk.38 rescues the small pupae phenotype seen in Nf1^E2/Nf1^E1 mutants.

Expression of Nf1^Scer\UAS.cWa under the control of the mushroom body drivers Scer\GAL4^c747, Scer\GAL4^Mef2.247 or Scer\GAL4^ey-OK107, and restricted to the adult stages using Scer\GAL80^ts.αTub84B, fails to rescue the olfactory learning defects seen in Nf1^E2/Nf1^E1 mutants.

Expression of Nf1^Scer\UAS.cWa under the control of the mushroom body drivers Scer\GAL4^elav-C155, Scer\GAL4^Ras64B.PW or Scer\GAL4^Alk.38, and restricted to the adult stages using Scer\GAL80^ts.αTub84B, fails to rescue the olfactory learning defects seen in Nf1^E2/Nf1^E1 mutants.

Expression of Nf1[Scer\UAS.cWa] under the control of the pan-neuronal driver Scer\GAL4^elav-C155, as well as Scer\GAL80^Mef2.PT to prevent expression in the mushroom bodies, rescues the olfactory learning defects seen in Nf1^E2/Nf1^E1 animals. Significant rescue is also seen with the Scer\GAL4^Ras64B.PW and Scer\GAL4^Alk.38 drivers.

(Gouzi et al., 2011)

The Nf1^E1/Nf1^E2 pupal and adult wing size defects are not rescued by expression of Nf1^Scer\UAS.cWa under the control of Scer\GAL4^en-e16E.

Expression of Nf1^Scer\UAS.cWa under the control of Scer\GAL4^elav-C155 strongly rescues the reduced pupal size and wing size defects of Nf1^E1/Nf1^E2 animals.

(Walker et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Nf1^Scer\UAS.cWa

Nf1^UAS.cWa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Walker

Secondary FlyBase IDs

References (5)

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