Expression of TrpA1^JF02461 under the control of Scer\GAL4^ppk.PG leads to abolished rolling behavior in response to heat stimulus compared to controls.

Expression of TrpA1^JF02461 under the control of Scer\GAL4^GMR60F07, Scer\GAL4^386Y, or the combination of Scer\GAL4^TrpA1-AB-G4 and Scer\GAL80^tsh-GAL80, but not Scer\GAL4^GMR21E09, Scer\GAL4^GMR21G01, or Scer\GAL4^GMR21F01, in combination with a Dicer-2 transgene to enhance RNAi efficiency, in larvae results in significantly reduced rolling (escape) behavior in response to noxious temperature increases, compared to controls.

Expression of TrpA1^JF02461 under the control of any of the following: Scer\GAL4^Rh5-G, Scer\GAL4^Rh6-G, Scer\GAL4^TrpA1-AB-G4 or Scer\GAL4^TrpA1-CD-G4 results in impaired thermotactic behavior in late third instar larvae (120 hr AEL): unlike controls, they display no strong preference for cooler temperatures (18[o]C) in a temperature gradient. Expression under Scer\GAL4^trp.PP has no significant effect on the larval thermal preferences.