FlyBase Reference Report: Ni et al., 2011.7.19, TRiP germline vectors pVALIUM20, pVALIUM21 and pVALIUM22.

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Ni, J.Q., Zhou, R., Czech, B., Liu, L.P., Holderbaum, L., Yang-Zhou, D., Shim, H.S., Handler, D., Karpowicz, P., Binari, R., Booker, M., Brennecke, J., Perkins, L.A., Hannon, G.J., Perrimon, N. (2011.7.19). TRiP germline vectors pVALIUM20, pVALIUM21 and pVALIUM22.

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FBrf0214641

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Personal communication to FlyBase

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VALIUM21, a variant of VALIUM22, differs only in that it lacks the ftz intron and gypsy sequences found in VALIUM22. VALIUM22 and VALIUM21 trigger effective RNAi knock-down and corresponding phenotypes in the female germline. The P-element transposase promoter is less effective than the hsp70 basal promoter in driving expression in somatic cells. Only 95 TRiP stocks have been generated in the VALIUM21 vector.
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See previous communication for description of VALIUM20 (FBrf0212437).

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English

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