FlyBase Allele Report: Scer\GAL4[TrpA1-AB-G4]

General Information

Symbol

Scer\GAL4^TrpA1-AB-G4

Species

S. cerevisiae

Name

FlyBase ID

FBal0324815

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

TI{GAL4}TrpA1^-AB-G4

Carried in Construct

Also Known As

trpA1^GAL4, trpA1-AB^GAL4, TRPA1^KI-GAL4

Allele class

Mutagen

ends-out gene targeting

Nature of the Allele

Allele class

Mutagen

ends-out gene targeting

(Kim et al., 2010)

Progenitor genotype

Associated Insertion(s)

TI{GAL4}TrpA1^-AB-G4

Cytology

Description

Deletion that spans the translation initiation codon common to the A and B isoforms of TrpA1, preventing expression of these two isoforms. Sequence encoding a GAL4 driver and a w^+* marker have been inserted in place of the deleted sequence. The GAL4 driver acts as a reporter of expression of the TrpA1 A and B isoforms.

(Luo et al., 2017)

Sequence encoding a GAL4 driver has been inserted at the site of the normal TrpA1 translation initiation codon, together with the simultaneous deletion of a 185 bp region encoding the N-terminal 61 residues of TrpA1.

(Kim et al., 2010)

Allele components

Component

Use(s)

Inserted element

TI{GAL4}

promoter trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

Additional Information

Statement

Reference

ScerGAL4^TrpA1-AB-G4 drives expression under control of the promoter for the TrpA1-RI and TrpA1-RJ (TrpA1-A and TrpA1-B; downstream promoter) isoforms.

(Bloomington Drosophila Stock Center, 2017)

TrpA1 did not co-localize in cells expressing Pdf in the morning cells, or Ilp2 in the pars intercerebralis.

(Lee, 2013)

ScerGAL4^TrpA1-AB-G4 drives expression in many pacemaker neurons.

(Lee and Montell, 2013)

Marker for

Reflects expression of

TrpA1

(Bloomington Drosophila Stock Center, 2017, Lee, 2013, Lee and Montell, 2013, Ni et al., 2013)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with red light) of Scer\GAL4^TrpA1-AB-G4 cells or a subset of Scer\GAL4^TrpA1-AB-G4 cells (restricted using Scer\GAL80^tsh-GAL80) in larvae results in a significant induction of nociceptive rolling behavior in the absence of nociceptive stimuli, compared to controls.

(Luo et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions