FlyBase Allele Report: Scer\GAL4[NP6024]

General Information

Symbol

Scer\GAL4^NP6024

Species

S. cerevisiae

Name

FlyBase ID

FBal0245050

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

P{GawB}NP6024

Carried in Construct

Also Known As

NP6024, NP6024-Gal4

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{GawB}NP6024

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{GawB}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

X:2,796,429..2,796,528 [+]

(Wong et al., 2023, Vrontou et al., 2021, Hamid et al., 2019, Flybrain Neuron Database et al., 2018.6.28, Groschner et al., 2018, Yagi et al., 2016, Gombos et al., 2015, DasGupta et al., 2014, Perisse et al., 2013, Xie et al., 2013, Huang et al., 2012, Pauls et al., 2010, Pauls et al., 2010, Tanaka et al., 2008)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

inner layer of the larval mushroom body

(Pauls et al., 2010, Pauls et al., 2010)

outer layer of the larval mushroom body

(Pauls et al., 2010, Pauls et al., 2010)

core layer of the larval mushroom body

(Pauls et al., 2010, Pauls et al., 2010)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal chemosensory behavior | adult stage, with Shal^2.UAS.GFP

(Groschner et al., 2018)

abnormal eclosion, with ChT^KK109385

(Hamid et al., 2019)

abnormal locomotor behavior | larval stage, with ChT^KK109385

(Hamid et al., 2019)

abnormal memory, with hiw^GD14101

(Huang et al., 2012)

abnormal memory, with hiw^ΔRING.UAS

(Huang et al., 2012)

abnormal memory | adult stage | heat sensitive, with shi^1.UAS

(Perisse et al., 2013, Xie et al., 2013)

increased mortality during development, with ChT^KK109385

(Hamid et al., 2019)

Phenotype Manifest In

Detailed Description

Statement

Reference

Blocking synaptic transmission (using shi^1.Scer\UAS at restrictive temperatures) from Scer\GAL4^NP6024 neurons significantly impairs sucrose-reinforced appetitive or electric-shock-reinforced aversive memory performance (3 hours after conditioning) compared to controls. Flies undergo conditioning at a permissive temperature (23[o]C), whereas testing is at a restrictive temperature (33[o]C); there are no significant memory differences when training and testing is at permissive temperatures. Scer\GAL4^NP6024>shi^1.Scer\UAS flies exhibit normal olfactory acuity at the restrictive temperature.

(Perisse et al., 2013)

Inhibition (using shi^1.UAS at 33[o]C) of Scer\GAL4^NP6024 cells in adults during testing significantly reduces 3 h appetitive memory, compared to controls.

(Xie et al., 2013)

Blocking synaptic transmission in Scer\GAL4^NP6024-expressing neurons for the 12 hour interval 6 hours after spaced training impairs aversive long term memory.

Blocking synaptic transmission in Scer\GAL4^NP6024-expressing neurons following starvation impairs appetitive long term memory (association of an odor with sucrose). Sensorimotor abilities are unchanged compared with controls.

(Huang et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

ChT^UAS.cHa/Scer\GAL4^NP6024 partially rescues ChT^KK109385

(Hamid et al., 2019)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

105080

y^* w^* P{GawB}NP6024 / FM7c

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^NP6024

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

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