FlyBase Allele Report: Dmel\Gprk2[KO]

General Information

Symbol

Dmel\Gprk2^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0245156

Feature type

allele

Associated gene

Dmel\Gprk2

Associated Insertion(s)

TI{TI}Gprk2^KO

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

recombination

(Cheng et al., 2010)

I-SceI

(Cheng et al., 2010)

FLPase

(Cheng et al., 2010)

ends-out gene targeting

(Cheng et al., 2010)

Progenitor genotype

Associated Insertion(s)

TI{TI}Gprk2^KO

Cytology

Description

Part of the Gprk2 locus has been replaced by a w^+mW.hs marker.

(Cheng et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of alcohol use disorder

CEA

(Kang et al., 2020)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

partially lethal - majority die | heat sensitive

(Cheng et al., 2010)

visible | heat sensitive

(Cheng et al., 2010)

Phenotype Manifest In

wing cell, with Df(3R)Gprk2-del1

(Cheng et al., 2012)

wing cell | heat sensitive (with Df(3R)Gprk2)

(Cheng et al., 2010)

wing vein L3 | heat sensitive (with Df(3R)Gprk2)

(Cheng et al., 2010)

wing vein L4 | heat sensitive (with Df(3R)Gprk2)

(Cheng et al., 2010)

Detailed Description

Statement

Reference

The L3-L4 intervein area as a fraction of total wing area in Gprk2^KO/Df(3R)Gprk2-del1 mutant flies raised at 27[o]C is reduced slightly compared to wild type.

(Cheng et al., 2012)

Gprk2^KO homozygotes are viable at 25[o]C, and semi-lethal at 29[o]C.

Gprk2^KO/Df(3R)Gprk2 animals raised at 29[o]C show smaller wings with reduced area between wing vein L3 and L4. This distance is normal in flies raised at 25[o]C.

(Cheng et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Gprk1^KO, Gprk2^KO has lethal phenotype

(Cheng et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Df(3R)Gprk2-del1, Gprk2^KO has wing cell phenotype, enhanceable by Pka-C1[+]/Pka-C1^H2

(Cheng et al., 2012)

Df(3R)Gprk2-del1, Gprk2^KO has wing cell phenotype, enhanceable by Pka-C1[+]/Pka-C1^DN

(Cheng et al., 2012)

Other

Statement

Reference

Gprk1^KO, Gprk2^KO has wing phenotype

(Cheng et al., 2010)

Gprk1[+]/Gprk1^KO, Gprk2^KO has wing vein L3 phenotype

(Cheng et al., 2010)

Gprk1[+]/Gprk1^KO, Gprk2^KO has wing cell phenotype

(Cheng et al., 2010)

Gprk1[+]/Gprk1^KO, Gprk2^KO has wing vein L4 phenotype

(Cheng et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of Pka-C1^H2 enhances the reduction in L3-L4 intervein area seen in Gprk2^KO/Df(3R)Gprk2-del1 mutant flies.

One copy of Pka-C1^DN enhances the reduction in L3-L4 intervein area seen in Gprk2^KO/Df(3R)Gprk2-del1 mutant flies.

(Cheng et al., 2012)

No Gprk1^KO/Gprk2^KO double-heterozygous adults are recovered.

Gprk1^KO/Gprk1^KO, Gprk2^KO/+ flies have smaller wing size but no reduction in L3-L4 intervein spacing at 25[o]C.

Gprk1^KO/+, Gprk2^KO/Gprk2^KO flies show a reduction in the L3-L4 intervein spacing at 25[o]C.

(Cheng et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Gprk2^KO

(Cheng et al., 2010)

gprk2 KO

(Giordano et al., 2018)

gprk2^KO

(Kang et al., 2020, Maier et al., 2014, Ranieri et al., 2014, Cheng et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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