The expression of N^JF01356 under the control of Scer\GAL4^109-30, in combination with gal80[ts] to restrict expression to adulthood, leads to defects in follicle cell differentiation and in basal stalk formation, resulting in partially fused egg chambers, as compared to controls.

Expression of one copy of N^JF01356 under the control of one copy of Scer\GAL4^bbg-C96 results in wing phenotypes that range from mild loss of margin bristles to strong wing notching. These phenotypes are more severe in males than females, and at 29[o]C than 25[o]C. A stronger phenotype is seen when two copies of the transgene are expressed, and doubling the dose of Scer\GAL4^bbg-C96 also increases the proportion of flies with severe wing notching phenotypes. However, little difference is seen between flies expressing either one or two copies of the transgene and two copies of Scer\GAL4^bbg-C96 (at 25[o]C). When one copy of Dcr-2^Scer\UAS.cDa is co-expressed with one copy each of N^JF01356 and Scer\GAL4^bbg-C96 the wing phenotypes are enhanced, but not as significantly as when the dose of Scer\GAL4^bbg-C96 is doubled.